Tryparedoxin peroxidase-deficiency commits trypanosomes to ferroptosis-type cell death

• Verfasst von: Bogacz, Marta [VerfasserIn]
• Verfasst von: Krauth-Siegel, Renate [VerfasserIn]
• Titel: Tryparedoxin peroxidase-deficiency commits trypanosomes to ferroptosis-type cell death
• Verf.angabe: Marta Bogacz, R Luise Krauth-Siegel
• E-Jahr: 2018
• Jahr: Jul 26, 2018
• Umfang: 29 S.
• Fussnoten: Gesehen am 07.04.2020
• Titel Quelle: Enthalten in: eLife
• Ort Quelle: Cambridge : eLife Sciences Publications, 2012
• Jahr Quelle: 2018
• Band/Heft Quelle: 7(2018) Artikel-Nummer e 37503, 29 Seiten
• ISSN Quelle: 2050-084X
• DOI: doi:10.7554/eLife.37503
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: ferroptosis
• Sach-SW: Trypanosoma brucei
• Sach-SW: trypanothione
• Sach-SW: tryparedoxin peroxidase
• K10plus-PPN: 1694151913

Abstract

Tryparedoxin peroxidases, distant relatives of glutathione peroxidase 4 in higher eukaryotes, are responsible for the detoxification of lipid-derived hydroperoxides in African trypanosomes. The lethal phenotype of procyclic Trypanosoma brucei that lack the enzymes fulfils all criteria defining a form of regulated cell death termed ferroptosis. Viability of the parasites is preserved by α-tocopherol, ferrostatin-1, liproxstatin-1 and deferoxamine. Without protecting agent, the cells display, primarily mitochondrial, lipid peroxidation, loss of the mitochondrial membrane potential and ATP depletion. Sensors for mitochondrial oxidants and chelatable iron as well as overexpression of a mitochondrial iron-superoxide dismutase attenuate the cell death. Electron microscopy revealed mitochondrial matrix condensation and enlarged cristae. The peroxidase-deficient parasites are subject to lethal iron-induced lipid peroxidation that probably originates at the inner mitochondrial membrane. Taken together, ferroptosis is an ancient cell death program that can occur at individual subcellular membranes and is counterbalanced by evolutionary distant thiol peroxidases.