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Verfasst von:Matsuzaki, Takahisa [VerfasserIn]   i
 Chevyreva, Veronika [VerfasserIn]   i
 Kaufmann, Stefan [VerfasserIn]   i
 Tanaka, Motomu [VerfasserIn]   i
Titel:Adsorption of galloyl catechin aggregates significantly modulates membrane mechanics in the absence of biochemical cues
Verf.angabe:Takahisa Matsuzaki, Hiroaki Ito, Veronika Chevyreva, Ali Makky, Stefan Kaufmann, Kazuki Okano, Naritaka Kobayashi, Masami Suganuma, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa and Motomu Tanaka
E-Jahr:2017
Jahr:07 Jul 2017
Umfang:11 S.
Fussnoten:Gesehen am 14.04.2020
Titel Quelle:Enthalten in: Physical chemistry, chemical physics
Ort Quelle:Cambridge : RSC Publ., 1999
Jahr Quelle:2017
Band/Heft Quelle:19(2017), 30, Seite 19937-19947
ISSN Quelle:1463-9084
Abstract:Physical interactions of four major green tea catechin derivatives with cell membrane models were systemically investigated. Catechins with the galloyl moiety caused the aggregation of small unilamellar vesicles and an increase in the surface pressure of lipid monolayers, while those without did not. Differential scanning calorimetry revealed that, in a low concentration regime (≤10 μM), catechin molecules are not significantly incorporated into the hydrophobic core of lipid membranes as substitutional impurities. Partition coefficient measurements revealed that the galloyl moiety of catechin and the cationic quaternary amine of lipids dominate the catechin-membrane interaction, which can be attributed to the combination of electrostatic and cation-π interactions. Finally, we shed light on the mechanical consequence of catechin-membrane interactions using the Fourier-transformation of the membrane fluctuation. Surprisingly, the incubation of cell-sized vesicles with 1 μM galloyl catechins, which is comparable to the level in human blood plasma after green tea consumption, significantly increased the bending stiffness of the membranes by a factor of more than 60, while those without the galloyl moiety had no detectable influence. Atomic force microscopy and circular dichroism spectroscopy suggest that the membrane stiffening is mainly attributed to the adsorption of galloyl catechin aggregates to the membrane surfaces. These results contribute to our understanding of the physical and thus the generic functions of green tea catechins in therapeutics, such as cancer prevention.
DOI:doi:10.1039/C7CP02771K
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1039/C7CP02771K
 Volltext: https://pubs.rsc.org/en/content/articlelanding/2017/cp/c7cp02771k
 DOI: https://doi.org/10.1039/C7CP02771K
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1694490106
Verknüpfungen:→ Zeitschrift

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