Multi-centre technical evaluation of the radiation-induced lymphocyte apoptosis assay as a predictive test for radiotherapy toxicity

• Verfasst von: Talbot, Christopher J. [VerfasserIn]
• Verfasst von: Veldwijk, Marlon Romano [VerfasserIn]
• Verfasst von: Bierbaum, Miriam [VerfasserIn]
• Verfasst von: Keller, Anke [VerfasserIn]
• Verfasst von: Chang-Claude, Jenny [VerfasserIn]
• Verfasst von: Sperk, Elena [VerfasserIn]
• Verfasst von: Wenz, Frederik [VerfasserIn]
• Verfasst von: Herskind, Carsten [VerfasserIn]
• Titel: Multi-centre technical evaluation of the radiation-induced lymphocyte apoptosis assay as a predictive test for radiotherapy toxicity
• Verf.angabe: Christopher J. Talbot, Marlon R. Veldwijk, David Azria, Chiara Batini, Miriam Bierbaum, Muriel Brengues, Jenny Chang-Claude, Kerstie Johnson, Anke Keller, Sheila Smith, Elena Sperk, R. Paul Symonds, Frederik Wenz, Catharine M.L. West, Carsten Herskind, Celine Bourgier
• E-Jahr: 2019
• Jahr: 10 June 2019
• Umfang: 8 S.
• Fussnoten: Gesehen am 16.04.2020
• Titel Quelle: Enthalten in: Clinical and translational radiation oncology
• Ort Quelle: Amsterdam : Elsevier, 2016
• Jahr Quelle: 2019
• Band/Heft Quelle: 18(2019), Seite 1-8
• ISSN Quelle: 2405-6308
• DOI: doi:10.1016/j.ctro.2019.06.001
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1694670082

Abstract

Predicting which patients will develop adverse reactions to radiotherapy is important for personalised treatment. Prediction will require an algorithm or nomogram combining clinical and biological data. The radiation-induced lymphocyte apoptosis (RILA) assay is the leading candidate as a biological predictor of radiotherapy toxicity. In this study we tested the potential of the assay for standardisation and use in multiple testing laboratories.</p><p>The assay was standardised and reproducibility determined using samples from healthy volunteers assayed concurrently in three laboratories in Leicester (UK), Mannheim (Germany) and Montpellier (France). RILA assays were performed on samples taken prior to radiotherapy from 1319 cancer patients enrolled in the REQUITE project at multiple centres. The patients were being treated for breast (n = 753), prostate (n = 506) or lung (n = 60) cancer.</p><p>Inter-laboratory comparisons identified several factors affecting results: storage time, incubation periods and type of foetal calf serum. Following standardisation, there was no significant difference in results between the centres. Significant differences were seen in RILA scores between cancer types (prostate > breast > lung), by smoking status (non-smokers > smokers) and co-morbidity with rheumatoid arthritis (arthritics > non-arthritics).</p><p>An analysis of acute radiotherapy toxicity showed as expected that RILA assay does not predict most end-points, but unexpectedly did predict acute breast pain. This result may elucidate the mechanism by which the RILA assay predicts late radiotherapy toxicity.</p><p>The work shows clinical trials involving multiple laboratory measurement of the RILA assay are feasible and the need to account for tumour type and other variables when applying to predictive models.</p>