| |  Online-Ressource |
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| Verfasst von: | Filipiak, Marcin Szymon [VerfasserIn]  |
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| | Rother, Marcel [VerfasserIn] |
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| | Andoy, Nesha M. [VerfasserIn] |
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| | Knudsen, Arne [VerfasserIn] |
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| | Grimm, Stefan [VerfasserIn] |
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| | Bachran, Christopher [VerfasserIn] |
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| | Swee, Lee Kim [VerfasserIn] |
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| | Zaumseil, Jana [VerfasserIn] |
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| | Tarasov, Alexey [VerfasserIn] |
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| Titel: | Highly sensitive, selective and label-free protein detection in physiological solutions using carbon nanotube transistors with nanobody receptors |
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| Verf.angabe: | Marcin S. Filipiak, Marcel Rother, Nesha M. Andoy, Arne C. Knudsen, Stefan Grimm, Christopher Bachran, Lee Kim Swee, Jana Zaumseil, Alexey Tarasov |
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| Jahr: | 2018 |
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| Umfang: | 10 S. |
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| Fussnoten: | Available online 24 August 2017 ; Gesehen am 17.04.2020 |
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| Titel Quelle: | Enthalten in: Sensors and actuators <Lausanne> / B |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1990 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 255(2018), Seite 1507-1516 |
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| ISSN Quelle: | 1873-3077 |
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| Abstract: | Nanomaterial-based field-effect transistors (FETs) have been proposed for real-time, label-free detection of various biological species. However, two major challenges have limited their use in physiological samples: screening of the analyte charge by electrolyte ions (Debye screening) and non-specific adsorption. Here, these challenges are overcome by combining highly stable FETs based on single-walled semiconducting carbon nanotube (SWCNTs) networks with a novel surface functionalization comprising: 1) short nanobody (VHH) receptors, and 2) a polyethylene glycol (PEG) layer. Nanobodies are stable, easy-to-produce biological receptors that are very small (∼2-4nm), thus enabling analyte binding closer to the sensor surface. Despite their unique properties, nanobodies have not been used yet as receptors in FET based biosensors. The addition of PEG strongly enhances the signal in high ionic strength environment. Using green fluorescent protein (GFP) as a model antigen, high selectivity and sub-picomolar detection limit with a dynamic range exceeding 5 orders of magnitude is demonstrated in physiological solutions. In addition, long-term stability measurements reveal a low drift of SWCNTs of 0.05mV/h. The presented immunoassay is fast, label-free, does not require any sample pretreatment or washing steps. |
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| DOI: | doi:10.1016/j.snb.2017.08.164 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.snb.2017.08.164 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0925400517315964 |
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| | DOI: https://doi.org/10.1016/j.snb.2017.08.164 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Debye screening |
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| | Field-effect transistor (FET) |
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| | Label-free immunosensing |
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| | Nanobodies (VHH) |
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| | Polyethylene glycol (PEG) |
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| | Single-walled carbon nanotube (SWCNT) networks |
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| K10plus-PPN: | 1694851222 |
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| Verknüpfungen: | → Zeitschrift |
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Highly sensitive, selective and label-free protein detection in physiological solutions using carbon nanotube transistors with nanobody receptors / Filipiak, Marcin Szymon [VerfasserIn]; 2018 (Online-Ressource)
