Status: Bibliographieeintrag
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| |  Online-Ressource |
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| Verfasst von: | Mund, Markus [VerfasserIn]  |
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| | Hoess, Philipp [VerfasserIn] |
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| Titel: | Systematic nanoscale analysis of endocytosis links efficient vesicle formation to patterned actin nucleation |
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| Verf.angabe: | Markus Mund, Johannes Albertus van der Beek, Joran Deschamps, Serge Dmitrieff, Philipp Hoess, Jooske Louise Monster, Andrea Picco, François Nédélec, Marko Kaksonen, Jonas Ries |
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| E-Jahr: | 2018 |
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| Jahr: | July 26, 2018 |
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| Umfang: | 31 S. |
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| Fussnoten: | Gesehen am 20.04.2020 |
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| Titel Quelle: | Enthalten in: Cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1974 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 174(2018), 4, Seite 884-896,e1-e17 |
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| ISSN Quelle: | 1097-4172 |
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| Abstract: | Clathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven by a self-assembled macromolecular machine of over 50 different proteins in tens to hundreds of copies. How these proteins are organized to produce endocytic vesicles with high precision and efficiency is not understood. Here, we developed high-throughput superresolution microscopy to reconstruct the nanoscale structural organization of 23 endocytic proteins from over 100,000 endocytic sites in yeast. We found that proteins assemble by radially ordered recruitment according to function. WASP family proteins form a circular nanoscale template on the membrane to spatially control actin nucleation during vesicle formation. Mathematical modeling of actin polymerization showed that this WASP nano-template optimizes force generation for membrane invagination and substantially increases the efficiency of endocytosis. Such nanoscale pre-patterning of actin nucleation may represent a general design principle for directional force generation in membrane remodeling processes such as during cell migration and division. |
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| DOI: | doi:10.1016/j.cell.2018.06.032 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.cell.2018.06.032 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0092867418308006 |
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| | DOI: https://doi.org/10.1016/j.cell.2018.06.032 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | actin |
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| | Brownian dynamics simulations |
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| | clathrin |
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| | endocytosis |
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| | high-throughput |
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| | single-molecule localization microscopy |
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| | SMLM |
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| | superresolution microscopy |
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| | WASP |
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| K10plus-PPN: | 1694964175 |
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| Verknüpfungen: | → Zeitschrift |
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Systematic nanoscale analysis of endocytosis links efficient vesicle formation to patterned actin nucleation / Mund, Markus [VerfasserIn]; July 26, 2018 (Online-Ressource)
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