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| Verfasst von: | Friedel, Thorsten [VerfasserIn]  |
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| | Jung-Klawitter, Sabine [VerfasserIn] |
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| | Sebe, Attila [VerfasserIn] |
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| | Schenk, Franziska [VerfasserIn] |
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| | Modlich, Ute [VerfasserIn] |
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| | Ivics, Zoltán [VerfasserIn] |
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| | Schumann, Gerald G. [VerfasserIn] |
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| | Buchholz, Christian J. [VerfasserIn] |
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| | Schneider, Irene C. [VerfasserIn] |
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| Titel: | CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification |
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| Verf.angabe: | Thorsten Friedel, Sabine Jung-Klawitter, Attila Sebe, Franziska Schenk, Ute Modlich, Zoltán Ivics, Gerald G. Schumann, Christian J. Buchholz and Irene C. Schneider |
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| E-Jahr: | 2016 |
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| Jahr: | 9 March 2016 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 21.04.2020 |
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| Titel Quelle: | Enthalten in: Stem Cells and Development |
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| Ort Quelle: | Larchmont, NY : Liebert, 2004 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 25(2016), 9, Seite 729-739 |
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| ISSN Quelle: | 1557-8534 |
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| Abstract: | Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstrate that CD30 expression is restricted to SSEA4high cells of human iPSC cultures and a human embryonic stem cell line. When CD30-LV was added to iPSCs during routine cultivation, efficient and exclusive transduction of cells positive for the pluripotency marker Oct-4 was achieved, while retaining their pluripotency. When added during the reprogramming process, CD30-LV solely transduced cells that became fully reprogrammed iPSCs as confirmed by co-expression of endogenous Nanog and the reporter gene. Thus, CD30-LV may serve as novel tool for the selective gene transfer into PSCs with broad applications in basic and therapeutic research. |
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| DOI: | doi:10.1089/scd.2015.0386 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1089/scd.2015.0386 |
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| | Volltext: https://www.liebertpub.com/doi/10.1089/scd.2015.0386 |
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| | DOI: https://doi.org/10.1089/scd.2015.0386 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1695272595 |
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| Verknüpfungen: | → Zeitschrift |
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CD30 receptor-targeted lentiviral vectors for human induced pluripotent stem cell-specific gene modification / Friedel, Thorsten [VerfasserIn]; 9 March 2016 (Online-Ressource)
