| |  Online-Ressource |
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| Verfasst von: | Mishra, Neha [VerfasserIn]  |
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| | Schwerdtner, Lidja [VerfasserIn] |
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| | Sams, Kelly [VerfasserIn] |
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| | Mondal, Santanu [VerfasserIn] |
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| | Ahmad, Fareed [VerfasserIn] |
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| | Schmidt, Reinhold E. [VerfasserIn] |
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| | Coonrod, Scott A. [VerfasserIn] |
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| | Thompson, Paul R. [VerfasserIn] |
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| | Lerch, Markus M. [VerfasserIn] |
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| | Bossaller, Lukas Friedrich Magnus [VerfasserIn] |
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| Titel: | Cutting Edge |
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| Titelzusatz: | Protein Arginine Deiminase 2 and 4 Regulate NLRP3 Inflammasome-Dependent IL-1β Maturation and ASC Speck Formation in Macrophages |
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| Verf.angabe: | Neha Mishra, Lidja Schwerdtner, Kelly Sams, Santanu Mondal, Fareed Ahmad, Reinhold E. Schmidt, Scott A. Coonrod, Paul R. Thompson, Markus M. Lerch, Lukas Bossaller |
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| E-Jahr: | 2019 |
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| Jahr: | 10 July 2019 |
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| Fussnoten: | Prepublished online 10 July 2019 ; Gesehen am 21.04.2020 |
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| Titel Quelle: | Enthalten in: The journal of immunology |
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| Ort Quelle: | Bethesda, Md. : Soc., 1916 |
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| Jahr Quelle: | 2019 |
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| Band/Heft Quelle: | 203(2019), 4, Seite 795-800 |
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| ISSN Quelle: | 1550-6606 |
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| Abstract: | Protein arginine deiminase (PAD) enzymes catalyze the conversion of protein-bound arginine into citrulline, an irreversible posttranslational modification with loss of a positive charge that can influence protein-protein interactions and protein structure. Protein arginine deiminase activity depends on high intracellular calcium concentrations occurring in dying cells. In this study, we demonstrate that protein citrullination is common during pyroptotic cell death in macrophages and that inhibition of PAD enzyme activity by Cl-amidine, a pan-PAD inhibitor, blocks NLRP3 inflammasome assembly and proinflammatory IL-1β release in macrophages. Genetic deficiency of either PAD2 or PAD4 alone in murine macrophages does not impair IL-1β release; however, pharmacological inhibition or small interfering RNA knockdown of PAD2 within PAD4−/− macrophages does. Our results suggest that PAD2 and 4 activity in macrophages is required for optimal inflammasome assembly and IL-1β release, a finding of importance for autoimmune diseases and inflammation. |
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| DOI: | doi:10.4049/jimmunol.1800720 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.4049/jimmunol.1800720 |
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| | Volltext: https://www.jimmunol.org/content/203/4/795 |
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| | DOI: https://doi.org/10.4049/jimmunol.1800720 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1695281446 |
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| Verknüpfungen: | → Zeitschrift |
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