Changes in global translation elongation or initiation rates shape the proteome via the Kozak sequence

• Verfasst von: Acevedo, Julieta M. [VerfasserIn]
• Verfasst von: Hoermann, Bernhard [VerfasserIn]
• Verfasst von: Schlimbach, Tilo [VerfasserIn]
• Verfasst von: Teleman, Aurelio A. [VerfasserIn]
• Titel: Changes in global translation elongation or initiation rates shape the proteome via the Kozak sequence
• Verf.angabe: Julieta M. Acevedo, Bernhard Hoermann, Tilo Schlimbach & Aurelio A. Teleman
• E-Jahr: 2018
• Jahr: 05 March 2018
• Umfang: 12 S.
• Fussnoten: Gesehen am 22.04.2020
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2018
• Band/Heft Quelle: 8(2018) Artikel-Nummer 4018, 12 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/s41598-018-22330-9
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1695609433

Abstract

The sequence context surrounding the AUG start codon of an open reading frame - the ‘Kozak sequence’ - affects the probability with which a scanning ribosome will recognize the start codon and start translating there. A significant number of transcripts in animals such as Drosophila contain weak Kozak sequences. This is predicted to cause constitutively low translation of these transcripts. We study here the additional possibility that these mRNAs have weak Kozak sequences to allow for the regulation of their translation in response to stress or altered cellular signaling. We find that transcripts with weak Kozak sequences are less sensitive to drops in global elongation rates and more sensitive to drops in global initiation rates compared to transcripts with strong Kozak sequences. This provides a mechanism by which changes in these global translation parameters differentially affect different pools of mRNAs depending on their Kozak sequence, thereby shaping the proteome. Interestingly, mRNAs with weak Kozak sequences are enriched for genes involved in neurobiology, suggesting that they constitute a functional group that can be translationally co-regulated.