Gender-specific reference values for high-sensitivity cardiac troponin T and I in well-phenotyped healthy individuals and validity of high-sensitivity assay designation

• Verfasst von: Giannitsis, Evangelos [VerfasserIn]
• Verfasst von: Müller-Hennessen, Matthias [VerfasserIn]
• Verfasst von: Zeller, Tanja [VerfasserIn]
• Verfasst von: Schübler, Anna-Sophie [VerfasserIn]
• Verfasst von: Aurich, Matthias [VerfasserIn]
• Verfasst von: Biener, Moritz [VerfasserIn]
• Verfasst von: Vafaie, Mehrshad [VerfasserIn]
• Verfasst von: Stoyanov, Kiril M. [VerfasserIn]
• Verfasst von: Ochs, Marco [VerfasserIn]
• Verfasst von: Riffel, Johannes [VerfasserIn]
• Verfasst von: Mereles, Derliz [VerfasserIn]
• Verfasst von: Blankenberg, Stefan [VerfasserIn]
• Verfasst von: Katus, Hugo [VerfasserIn]
• Titel: Gender-specific reference values for high-sensitivity cardiac troponin T and I in well-phenotyped healthy individuals and validity of high-sensitivity assay designation
• Verf.angabe: Evangelos Giannitsis, Matthias Mueller-Hennessen, Tanja Zeller, Anna Schuebler, Matthias Aurich, Moritz Biener, Mehrshad Vafaie, Kiril M. Stoyanov, Marco Ochs, Johannes Riffel, Derliz Mereles, Stefan Blankenberg, Hugo A. Katus
• Jahr: 2020
• Umfang: 7 S.
• Fussnoten: Available online 28 November 2019 ; Gesehen am 27.04.2020
• Titel Quelle: Enthalten in: Clinical biochemistry
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1967
• Jahr Quelle: 2020
• Band/Heft Quelle: 78(2020), Seite 18-24
• ISSN Quelle: 1873-2933
• DOI: doi:10.1016/j.clinbiochem.2019.11.013
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cardiac troponin
• Sach-SW: Gender
• Sach-SW: Healthy cohort
• Sach-SW: High-sensitivity
• Sach-SW: Reference values
• K10plus-PPN: 1696246482

Abstract

Objective - To determine gender-specific reference limits of high-sensitivity (hs) cardiac troponins (cTn) and validity of hs assay designation for both genders. - Methods - After screening with a questionnaire, 827 presumably healthy individuals were further selected based on clinical criteria (n = 740), clinical criteria plus cardiac imaging including stress magnetic resonance imaging or stress echocardiography (n = 726), and extended cardio-pulmonary parameters (n = 626). Blood samples were measured with hs-cTnT (Roche Diagnostics) on a cobas e602 analyzer as well as hs-cTnI (Abbott Diagnostics) on an ARCHITECTi2000SR. The impact of health definition, statistical methods, instrument selection and limit of detection (LoD) on overall and gender-specific 99th percentiles was assessed. - Results - Median age was 56 years (50.9% female) for the total study cohort. 99th percentiles for females and males ranged between 13.1 and 13.3 ng/L and 16.8-19.9 ng/L for hs-cTnT as well as 10.3-12.5 ng/L and 27.4-29.7 ng/L for hs-cTnI depending on health definition. Utilization of stricter health definition criteria reduced the difference of the gender-specific 99th percentiles between males and females for hs-cTnT to 3.7 ng/L (males 16.8 ng/L, females 13.1 ng/L), whereas the difference rather increased for hs-cTnI to 19.4 ng/L (males 29.7 ng/L, females 10.3 ng/L). Values > LoD could be measured in the majority of males and females using hs-TnT (81.4-83.3% and 96.5-96.9%, respectively). In contrast, values > LoD could not be observed in the majority of females using hs-cTnI (38.4-41.1%). - Conclusions - In a well-phenotyped healthy cohort, reference values for hs-cTnT were slightly higher, whereas hs-cTnI cut-offs were considerably lower than previously observed. Gender differences were more pronounced in hs-cTnI than in hs-cTnT and were further reduced for hs-cTnT by application of stricter health definition criteria. Contrary to hs-cTnI, hs-cTnT fulfilled criteria for hs designation for both genders.