| |  Online-Ressource |
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| Verfasst von: | Dubash, Taronish Dorab [VerfasserIn]  |
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| | Hoffmann, Christopher M. [VerfasserIn] |
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| | Oppel, Felix [VerfasserIn] |
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| | Gießler, Klara Monika [VerfasserIn] |
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| | Weber, Sarah [VerfasserIn] |
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| | Dieter, Sebastian M. [VerfasserIn] |
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| | Hüllein, Jennifer [VerfasserIn] |
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| | Zenz, Thorsten [VerfasserIn] |
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| | Herbst, Friederike [VerfasserIn] |
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| | Scholl, Claudia [VerfasserIn] |
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| | Weichert, Wilko [VerfasserIn] |
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| | Werft, Wiebke [VerfasserIn] |
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| | Benner, Axel [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Schneider, Martin [VerfasserIn] |
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| | Glimm, Hanno [VerfasserIn] |
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| | Ball, Claudia R. [VerfasserIn] |
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| Titel: | Phenotypic differentiation does not affect tumorigenicity of primary human colon cancer initiating cells |
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| Verf.angabe: | Taronish D. Dubash, Christopher M. Hoffmann, Felix Oppel, Klara M. Giessler, Sarah Weber, Sebastian M. Dieter, Jennifer Hüllein, Thorsten Zenz, Friederike Herbst, Claudia Scholl, Wilko Weichert, Wiebke Werft, Axel Benner, Manfred Schmidt, Martin Schneider, Hanno Glimm, Claudia R. Ball |
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| Jahr: | 2016 |
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| Umfang: | 8 S. |
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| Fussnoten: | Available online 8 December 2015 ; Gesehen am 28.04.2020 |
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| Titel Quelle: | Enthalten in: Cancer letters |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1975 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 371(2016), 2, Seite 326-333 |
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| ISSN Quelle: | 1872-7980 |
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| Abstract: | Within primary colorectal cancer (CRC) a subfraction of all tumor-initiating cells (TIC) drives long-term progression in serial xenotransplantation. It has been postulated that efficient maintenance of TIC activity in vitro requires serum-free spheroid culture conditions that support a stem-like state of CRC cells. To address whether tumorigenicity is indeed tightly linked to such a stem-like state in spheroids, we transferred TIC-enriched spheroid cultures to serum-containing adherent conditions that should favor their differentiation. Under these conditions, primary CRC cells did no longer grow as spheroids but formed an adherent cell layer, up-regulated colon epithelial differentiation markers, and down-regulated TIC-associated markers. Strikingly, upon xenotransplantation cells cultured under either condition equally efficient formed serially transplantable tumors. Clonal analyses of individual lentivirally marked TIC clones cultured under either culture condition revealed no systematic differences in contributing clone numbers, indicating that phenotypic differentiation does not select for few individual clones adapted to unfavorable culture conditions. Our results reveal that CRC TIC can be propagated under conditions previously thought to induce their elimination. This phenotypic plasticity allows addressing primary human CRC TIC properties in experimental settings based on adherent cell growth. |
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| DOI: | doi:10.1016/j.canlet.2015.11.037 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.canlet.2015.11.037 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0304383515007272 |
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| | DOI: https://doi.org/10.1016/j.canlet.2015.11.037 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Colon cancer |
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| | Phenotypic differentiation |
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| | Tumor initiating cells |
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| K10plus-PPN: | 1696279666 |
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| Verknüpfungen: | → Zeitschrift |
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Phenotypic differentiation does not affect tumorigenicity of primary human colon cancer initiating cells / Dubash, Taronish Dorab [VerfasserIn]; 2016 (Online-Ressource)
