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| Verfasst von: | García Caballero, Gabriel [VerfasserIn]  |
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| | Kaltner, Herbert [VerfasserIn] |
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| | Michalak, Malwina [VerfasserIn] |
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| | Shilova, Nadezhda [VerfasserIn] |
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| | Yegres, Michelle [VerfasserIn] |
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| | André, Sabine [VerfasserIn] |
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| | Ludwig, Anna-Kristin [VerfasserIn] |
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| | Manning, Joachim C. [VerfasserIn] |
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| | Schmidt, Sebastian [VerfasserIn] |
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| | Schnölzer, Martina [VerfasserIn] |
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| | Bovin, Nicolai V. [VerfasserIn] |
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| | Reusch, Dietmar [VerfasserIn] |
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| | Kopitz, Jürgen [VerfasserIn] |
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| | Gabius, Hans-Joachim [VerfasserIn] |
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| Titel: | Chicken GRIFIN |
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| Titelzusatz: | a homodimeric member of the galectin network with canonical properties and a unique expression profile |
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| Verf.angabe: | Gabriel García Caballero, Herbert Kaltner, Malwina Michalak, Nadezhda Shilova, Michelle Yegres, Sabine André, Anna-Kristin Ludwig, Joachim C. Manning, Sebastian Schmidt, Martina Schnölzer, Nicolai V. Bovin, Dietmar Reusch, Jürgen Kopitz, Hans-Joachim Gabius |
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| E-Jahr: | 2016 |
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| Jahr: | 11 June 2016 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 28.04.2020 |
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| Titel Quelle: | Enthalten in: Biochimie |
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| Ort Quelle: | Paris [u.a.] : Elsevier, 1971 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 128-129(2016), Seite 34-47 |
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| Abstract: | Occurrence of the adhesion/growth-regulatory galectins as family sets the challenge to achieve a complete network analysis. Along this route taken for a well-suited model organism (chicken), we fill the remaining gap to characterize its seventh member known from rat as galectin-related inter-fiber protein (GRIFIN) in the lens. Its single-copy gene is common to vertebrates, with one or more deviations from the so-called signature sequence for ligand (lactose) contact. The chicken protein is a homodimeric agglutinin with capacity to bind β-galactosides, especially the histo-blood group B tetrasaccharide, shown by solid-phase/cell assays and a glycan microarray. Mass spectrometric identification of two lactose-binding peptides after tryptic on-bead fragmentation suggests an interaction at the canonical region despite a sequence change from Arg to Val at the site, which impairs reactivity of human galectin-1. RT-PCR and Western blot analyses of specimen from adult chicken organs reveal restriction of expression to the lens, here immunohistochemically throughout its main body. This report sets the stage for detailed structure-activity studies to define factors relevant for affinity beyond the signature sequence and to perform the first complete network analysis of the galectin family in developing and adult organs of a vertebrate. |
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| DOI: | doi:10.1016/j.biochi.2016.06.001 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.biochi.2016.06.001 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0300908416301109 |
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| | DOI: https://doi.org/10.1016/j.biochi.2016.06.001 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Fiber cells |
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| | In-source decay |
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| | Lectin |
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| | Lens |
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| | Phylogenesis |
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| K10plus-PPN: | 1696358876 |
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| Verknüpfungen: | → Zeitschrift |
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Chicken GRIFIN / García Caballero, Gabriel [VerfasserIn]; 11 June 2016 (Online-Ressource)
