| |  Online-Ressource |
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| Verfasst von: | Carvalho, Silvia L. [VerfasserIn]  |
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| | Catarino, T. A. [VerfasserIn] |
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| | Dias, A. M. [VerfasserIn] |
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| | Kato, M. [VerfasserIn] |
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| | Almeida, A. [VerfasserIn] |
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| | Heßling, Bernd [VerfasserIn] |
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| | Figueiredo, J. [VerfasserIn] |
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| | Gärtner, F. [VerfasserIn] |
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| | Sanches, J. M. [VerfasserIn] |
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| | Ruppert, Thorsten [VerfasserIn] |
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| | Miyoshi, E. [VerfasserIn] |
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| | Pierce, M. [VerfasserIn] |
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| | Carneiro, F. [VerfasserIn] |
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| | Kolarich, D. [VerfasserIn] |
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| | Seruca, R. [VerfasserIn] |
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| | Yamaguchi, Y. [VerfasserIn] |
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| | Taniguchi, N. [VerfasserIn] |
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| | Reis, C. A. [VerfasserIn] |
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| | Pinho, S. S. [VerfasserIn] |
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| Titel: | Preventing E-cadherin aberrant N -glycosylation at Asn-554 improves its critical function in gastric cancer |
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| Verf.angabe: | S. Carvalho, T.A. Catarino, A.M. Dias, M. Kato, A. Almeida, B. Hessling, J. Figueiredo, F. Gärtner, J.M. Sanches, T. Ruppert, E. Miyoshi, M. Pierce, F. Carneiro, D. Kolarich, R. Seruca, Y. Yamaguchi, N. Taniguchi, C.A. Reis and S.S. Pinho |
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| Jahr: | 2016 |
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| Jahr des Originals: | 2015 |
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| Umfang: | 13 S. |
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| Fussnoten: | Published 20 July 2015 ; Gesehen am 29.04.2020 |
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| Titel Quelle: | Enthalten in: Oncogene |
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| Ort Quelle: | London : Springer Nature, 1997 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 35(2016), 13, Seite 1619-1631 |
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| ISSN Quelle: | 1476-5594 |
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| Abstract: | E-cadherin is a central molecule in the process of gastric carcinogenesis and its posttranslational modifications by N-glycosylation have been described to induce a deleterious effect on cell adhesion associated with tumor cell invasion. However, the role that site-specific glycosylation of E-cadherin has in its defective function in gastric cancer cells needs to be determined. Using transgenic mice models and human clinical samples, we demonstrated that N-acetylglucosaminyltransferase V (GnT-V)-mediated glycosylation causes an abnormal pattern of E-cadherin expression in the gastric mucosa. In vitro models further indicated that, among the four potential N-glycosylation sites of E-cadherin, Asn-554 is the key site that is selectively modified with β1,6 GlcNAc-branched N-glycans catalyzed by GnT-V. This aberrant glycan modification on this specific asparagine site of E-cadherin was demonstrated to affect its critical functions in gastric cancer cells by affecting E-cadherin cellular localization, cis-dimer formation, molecular assembly and stability of the adherens junctions and cell-cell aggregation, which was further observed in human gastric carcinomas. Interestingly, manipulating this site-specific glycosylation, by preventing Asn-554 from receiving the deleterious branched structures, either by a mutation or by silencing GnT-V, resulted in a protective effect on E-cadherin, precluding its functional dysregulation and contributing to tumor suppression. |
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| DOI: | doi:10.1038/onc.2015.225 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/onc.2015.225 |
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| | Volltext: https://www.nature.com/articles/onc2015225 |
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| | DOI: https://doi.org/10.1038/onc.2015.225 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1696834171 |
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| Verknüpfungen: | → Zeitschrift |
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Preventing E-cadherin aberrant N -glycosylation at Asn-554 improves its critical function in gastric cancer / Carvalho, Silvia L. [VerfasserIn]; 2016 (Online-Ressource)
