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| Verfasst von: | Hanne, Janina [VerfasserIn]  |
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| | Göttfert, Fabian [VerfasserIn] |
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| | Schimer, Jiří [VerfasserIn] |
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| | Anders-Ößwein, Maria [VerfasserIn] |
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| | Konvalinka, Jan [VerfasserIn] |
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| | Engelhardt, Johann [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| | Hell, Stefan [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| Titel: | Stimulated emission depletion nanoscopy reveals time-course of human immunodeficiency virus proteolytic maturation |
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| Verf.angabe: | Janina Hanne, Fabian Göttfert, Jiří Schimer, Maria Anders-Össwein, Jan Konvalinka, Johann Engelhardt, Barbara Müller, Stefan W. Hell, Hans-Georg Kräusslich |
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| E-Jahr: | 2016 |
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| Jahr: | August 12, 2016 |
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| Umfang: | 8 S. |
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| Teil: | volume:10 |
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| | year:2016 |
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| | number:9 |
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| | pages:8215-8222 |
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| | extent:8 |
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| Fussnoten: | Gesehen am 30.04.2020 |
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| Titel Quelle: | Enthalten in: American Chemical SocietyACS nano |
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| Ort Quelle: | Washington, DC : Soc., 2007 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 10(2016), 9, Seite 8215-8222 |
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| ISSN Quelle: | 1936-086X |
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| Abstract: | Concomitant with human immunodeficiency virus type 1 (HIV-1) budding from a host cell, cleavage of the structural Gag polyproteins by the viral protease (PR) triggers complete remodeling of virion architecture. This maturation process is essential for virus infectivity. Electron tomography provided structures of immature and mature HIV-1 with a diameter of 120-140 nm, but information about the sequence and dynamics of structural rearrangements is lacking. Here, we employed super-resolution STED (stimulated emission depletion) fluorescence nanoscopy of HIV-1 carrying labeled Gag to visualize the virion architecture. The incomplete Gag lattice of immature virions was clearly distinguishable from the condensed distribution of mature protein subunits. Synchronized activation of PR within purified particles by photocleavage of a caged PR inhibitor enabled time-resolved in situ observation of the induction of proteolysis and maturation by super-resolution microscopy. This study shows the rearrangement of subviral structures in a super-resolution light microscope over time, outwitting phototoxicity and fluorophore bleaching through synchronization of a biological process by an optical switch. |
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| DOI: | doi:10.1021/acsnano.6b03850 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1021/acsnano.6b03850 |
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| | DOI: https://doi.org/10.1021/acsnano.6b03850 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1696923816 |
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| Verknüpfungen: | → Zeitschrift |
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Stimulated emission depletion nanoscopy reveals time-course of human immunodeficiency virus proteolytic maturation / Hanne, Janina [VerfasserIn]; August 12, 2016 (Online-Ressource)
