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| Verfasst von: | Billon, Pierre [VerfasserIn]  |
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| | Nambiar, Tarun S. [VerfasserIn] |
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| | Hayward, Samuel B. [VerfasserIn] |
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| | Zafra, Maria P. [VerfasserIn] |
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| | Schatoff, Emma M. [VerfasserIn] |
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| | Oshima, Koichi [VerfasserIn] |
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| | Dunbar, Andrew [VerfasserIn] |
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| | Breinig, Marco [VerfasserIn] |
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| | Park, Young C. [VerfasserIn] |
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| | Ryu, Han S. [VerfasserIn] |
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| | Tschaharganeh, Darjus-Felix [VerfasserIn] |
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| | Levine, Ross L. [VerfasserIn] |
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| | Baer, Richard [VerfasserIn] |
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| | Ferrando, Adolfo [VerfasserIn] |
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| | Dow, Lukas E. [VerfasserIn] |
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| | Ciccia, Alberto [VerfasserIn] |
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| Titel: | Detection of marker-free precision genome editing and genetic variation through the capture of genomic signatures |
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| Verf.angabe: | Pierre Billon, Tarun S. Nambiar, Samuel B. Hayward, Maria P. Zafra, Emma M. Schatoff, Koichi Oshima, Andrew Dunbar, Marco Breinig, Young C. Park, Han S. Ryu, Darjus F. Tschaharganeh, Ross L. Levine, Richard Baer, Adolfo Ferrando, Lukas E. Dow, and Alberto Ciccia |
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| E-Jahr: | 2020 |
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| Jahr: | March 10, 2020 |
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| Umfang: | 22 S. |
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| Fussnoten: | Gesehen am 30.04.2020 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 30(2020), 10, Seite 3280-3295,e1-e6 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Genome editing technologies have transformed our ability to engineer desired genomic changes within living systems. However, detecting precise genomic modifications often requires sophisticated, expensive, and time-consuming experimental approaches. Here, we describe DTECT (Dinucleotide signaTurE CapTure), a rapid and versatile detection method that relies on the capture of targeted dinucleotide signatures resulting from the digestion of genomic DNA amplicons by the type IIS restriction enzyme AcuI. DTECT enables the accurate quantification of marker-free precision genome editing events introduced by CRISPR-dependent homology-directed repair, base editing, or prime editing in various biological systems, such as mammalian cell lines, organoids, and tissues. Furthermore, DTECT allows the identification of oncogenic mutations in cancer mouse models, patient-derived xenografts, and human cancer patient samples. The ease, speed, and cost efficiency by which DTECT identifies genomic signatures should facilitate the generation of marker-free cellular and animal models of human disease and expedite the detection of human pathogenic variants. |
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| DOI: | doi:10.1016/j.celrep.2020.02.068 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.celrep.2020.02.068 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S2211124720302369 |
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| | DOI: https://doi.org/10.1016/j.celrep.2020.02.068 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | base editing |
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| | CRISPR |
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| | detection method |
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| | dinucleotide signatures |
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| | genetic variation |
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| | homology-directed repair |
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| | human pathogenic variants |
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| | precision genome editing |
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| | prime editing |
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| | type IIS restriction endonucleases |
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| K10plus-PPN: | 1696951364 |
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| Verknüpfungen: | → Zeitschrift |
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Detection of marker-free precision genome editing and genetic variation through the capture of genomic signatures / Billon, Pierre [VerfasserIn]; March 10, 2020 (Online-Ressource)
