Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Mochalov, S. V. [VerfasserIn]   i
 Tarasova, N. V. [VerfasserIn]   i
 Kudryashova, T. V. [VerfasserIn]   i
 Gaynullina, Dina [VerfasserIn]   i
 Kalenchuk, V. U. [VerfasserIn]   i
 Borovik, Anatoly S. [VerfasserIn]   i
 Vorotnikov, Alexander V. [VerfasserIn]   i
 Tarasova, Olga S. [VerfasserIn]   i
 Schubert, Rudolf [VerfasserIn]   i
Titel:Higher Ca2+ -sensitivity of arterial contraction in 1-week-old rats is due to a greater Rho-kinase activity
Verf.angabe:S.V. Mochalov, N.V. Tarasova, T.V. Kudryashova, D.K. Gaynullina, V.U. Kalenchuk, A.S. Borovik, A.V. Vorotnikov, O.S. Tarasova, R. Schubert
E-Jahr:2018
Jahr:31 January 2018
Umfang:15 S.
Fussnoten:Gesehen am 30.04.2020 ; Im Titel ist "2+" hochgestellt
Titel Quelle:Enthalten in: Acta physiologica
Ort Quelle:Oxford [u.a.] : Wiley-Blackwell, 2006
Jahr Quelle:2018
Band/Heft Quelle:223(2018,3) Artikel-Nummer e13044, 15 Seiten
ISSN Quelle:1748-1716
Abstract:AIM: During early post-natal development, arterial contraction depends less on Ca2+ -signalling pathways but more on changes in Ca2+ -sensitivity compared to adult animals. Whether this difference is related to Rho-kinase, one of the major players affecting Ca2+ -sensitivity, is unknown for intact vessels. Thus, we tested the hypothesis that Rho-kinase critically contributes to the higher Ca2+ -sensitivity of contraction in intact arteries of 1-week-old rats. - METHODS: We studied 1-week-old, 4- to 5-week-old and 10- to 12-week-old rats performing isometric myography, Ca2+ -fluorimetry and Western blotting using intact saphenous arteries and arterial pressure measurements under urethane anaesthesia. - RESULTS: In 10- to 12-week-old rats, methoxamine (MX) produced vasoconstriction associated with an increase in [Ca2+ ]i and Ca2+ -sensitivity. In contrast, in 1-week-old rats these contractions were accompanied only by an increase in Ca2+ -sensitivity. All MX-induced effects were reduced by the Rho-kinase inhibitor Y-27632; this reduction was complete only in 1-week-old rats. The Rho-kinase specific site Thr855 on MYPT1 was increasingly phosphorylated by MX in vessels of 1-week-old, but not 10- to 12-week-old rats; this effect was also inhibited completely by Y-27632. The Rho-kinase inhibitor fasudil in a dose not affecting the pressor response to MX in 4- to 5-week-old rats reduced it considerably in 1-week-old rats. - CONCLUSION: Our results suggest that the higher Ca2+ -sensitivity of arterial contraction in 1-week-old compared to 10- to 12-week-old rats is due to a greater Rho-kinase activity. Constitutively active Rho-kinase contributes to MX-induced contraction in 10- to 12-week-old rats. In 1-week-old rats, additional Rho-kinase activation is involved. This remodelling of the Rho-kinase pathway is associated with its increased contribution to adrenergic arterial pressure responses.
DOI:doi:10.1111/apha.13044
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1111/apha.13044
 DOI: https://doi.org/10.1111/apha.13044
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Aging
 Animals
 Arteries
 Calcium Signaling
 Male
 maturation
 Methoxamine
 Muscle, Smooth, Vascular
 MYPT1
 protein kinase C
 Protein Kinase C
 Rats, Wistar
 rho-Associated Kinases
 Rho-kinase
 small arteries
 smooth muscle
 Vasoconstriction
K10plus-PPN:1696951607
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68572223   QR-Code
zum Seitenanfang