| Online-Ressource |
Verfasst von: | Mochalov, S. V. [VerfasserIn]  |
| Tarasova, N. V. [VerfasserIn]  |
| Kudryashova, T. V. [VerfasserIn]  |
| Gaynullina, Dina [VerfasserIn]  |
| Kalenchuk, V. U. [VerfasserIn]  |
| Borovik, Anatoly S. [VerfasserIn]  |
| Vorotnikov, Alexander V. [VerfasserIn]  |
| Tarasova, Olga S. [VerfasserIn]  |
| Schubert, Rudolf [VerfasserIn]  |
Titel: | Higher Ca2+ -sensitivity of arterial contraction in 1-week-old rats is due to a greater Rho-kinase activity |
Verf.angabe: | S.V. Mochalov, N.V. Tarasova, T.V. Kudryashova, D.K. Gaynullina, V.U. Kalenchuk, A.S. Borovik, A.V. Vorotnikov, O.S. Tarasova, R. Schubert |
E-Jahr: | 2018 |
Jahr: | 31 January 2018 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 30.04.2020 ; Im Titel ist "2+" hochgestellt |
Titel Quelle: | Enthalten in: Acta physiologica |
Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 2006 |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 223(2018,3) Artikel-Nummer e13044, 15 Seiten |
ISSN Quelle: | 1748-1716 |
Abstract: | AIM: During early post-natal development, arterial contraction depends less on Ca2+ -signalling pathways but more on changes in Ca2+ -sensitivity compared to adult animals. Whether this difference is related to Rho-kinase, one of the major players affecting Ca2+ -sensitivity, is unknown for intact vessels. Thus, we tested the hypothesis that Rho-kinase critically contributes to the higher Ca2+ -sensitivity of contraction in intact arteries of 1-week-old rats. - METHODS: We studied 1-week-old, 4- to 5-week-old and 10- to 12-week-old rats performing isometric myography, Ca2+ -fluorimetry and Western blotting using intact saphenous arteries and arterial pressure measurements under urethane anaesthesia. - RESULTS: In 10- to 12-week-old rats, methoxamine (MX) produced vasoconstriction associated with an increase in [Ca2+ ]i and Ca2+ -sensitivity. In contrast, in 1-week-old rats these contractions were accompanied only by an increase in Ca2+ -sensitivity. All MX-induced effects were reduced by the Rho-kinase inhibitor Y-27632; this reduction was complete only in 1-week-old rats. The Rho-kinase specific site Thr855 on MYPT1 was increasingly phosphorylated by MX in vessels of 1-week-old, but not 10- to 12-week-old rats; this effect was also inhibited completely by Y-27632. The Rho-kinase inhibitor fasudil in a dose not affecting the pressor response to MX in 4- to 5-week-old rats reduced it considerably in 1-week-old rats. - CONCLUSION: Our results suggest that the higher Ca2+ -sensitivity of arterial contraction in 1-week-old compared to 10- to 12-week-old rats is due to a greater Rho-kinase activity. Constitutively active Rho-kinase contributes to MX-induced contraction in 10- to 12-week-old rats. In 1-week-old rats, additional Rho-kinase activation is involved. This remodelling of the Rho-kinase pathway is associated with its increased contribution to adrenergic arterial pressure responses. |
DOI: | doi:10.1111/apha.13044 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/apha.13044 |
| DOI: https://doi.org/10.1111/apha.13044 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Aging |
| Animals |
| Arteries |
| Calcium Signaling |
| Male |
| maturation |
| Methoxamine |
| Muscle, Smooth, Vascular |
| MYPT1 |
| protein kinase C |
| Protein Kinase C |
| Rats, Wistar |
| rho-Associated Kinases |
| Rho-kinase |
| small arteries |
| smooth muscle |
| Vasoconstriction |
K10plus-PPN: | 1696951607 |
Verknüpfungen: | → Zeitschrift |
Higher Ca2+ -sensitivity of arterial contraction in 1-week-old rats is due to a greater Rho-kinase activity / Mochalov, S. V. [VerfasserIn]; 31 January 2018 (Online-Ressource)