| |  Online-Ressource |
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| Verfasst von: | Eck, Simon [VerfasserIn]  |
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| | Wörz, Stefan [VerfasserIn] |
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| | Müller-Ott, Katharina [VerfasserIn] |
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| | Hahn, Matthias [VerfasserIn] |
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| | Biesdorf, Andreas [VerfasserIn] |
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| | Schotta, Gunnar [VerfasserIn] |
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| | Rippe, Karsten [VerfasserIn] |
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| | Rohr, Karl [VerfasserIn] |
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| Titel: | A spherical harmonics intensity model for 3D segmentation and 3D shape analysis of heterochromatin foci |
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| Verf.angabe: | Simon Eck, Stefan Wörz, Katharina Müller-Ott, Matthias Hahn, Andreas Biesdorf, Gunnar Schotta, Karsten Rippe, Karl Rohr |
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| E-Jahr: | 2016 |
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| Jahr: | 17 March 2016 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 05.05.2020 |
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| Titel Quelle: | Enthalten in: Medical image analysis |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1996 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 32(2016), Seite 18-31 |
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| ISSN Quelle: | 1361-8423 |
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| Abstract: | The genome is partitioned into regions of euchromatin and heterochromatin. The organization of heterochromatin is important for the regulation of cellular processes such as chromosome segregation and gene silencing, and their misregulation is linked to cancer and other diseases. We present a model-based approach for automatic 3D segmentation and 3D shape analysis of heterochromatin foci from 3D confocal light microscopy images. Our approach employs a novel 3D intensity model based on spherical harmonics, which analytically describes the shape and intensities of the foci. The model parameters are determined by fitting the model to the image intensities using least-squares minimization. To characterize the 3D shape of the foci, we exploit the computed spherical harmonics coefficients and determine a shape descriptor. We applied our approach to 3D synthetic image data as well as real 3D static and real 3D time-lapse microscopy images, and compared the performance with that of previous approaches. It turned out that our approach yields accurate 3D segmentation results and performs better than previous approaches. We also show that our approach can be used for quantifying 3D shape differences of heterochromatin foci. |
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| DOI: | doi:10.1016/j.media.2016.03.001 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.media.2016.03.001 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S1361841516300019 |
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| | DOI: https://doi.org/10.1016/j.media.2016.03.001 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 3D parametric intensity model |
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| | Confocal light microscopy |
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| | Pericentric heterochromatin |
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| | Spherical harmonics |
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| K10plus-PPN: | 1697182194 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ spherical harmonics intensity model for 3D segmentation and 3D shape analysis of heterochromatin foci / Eck, Simon [VerfasserIn]; 17 March 2016 (Online-Ressource)
