Mcp3 is a novel mitochondrial outer membrane protein that follows a unique IMP-dependent biogenesis pathway

• Verfasst von: Sinzel, Monika [VerfasserIn]
• Verfasst von: Özbalcı, Çağakan [VerfasserIn]
• Verfasst von: Brügger, Britta [VerfasserIn]
• Titel: Mcp3 is a novel mitochondrial outer membrane protein that follows a unique IMP-dependent biogenesis pathway
• Verf.angabe: Monika Sinzel, Tao Tan, Philipp Wendling, Hubert Kalbacher, Cagakan Özbalci, Xenia Chelius, Benedikt Westermann, Britta Brügger, Doron Rapaport & Kai Stefan Dimmer
• E-Jahr: 2016
• Jahr: 24 May 2016
• Umfang: 17 S.
• Fussnoten: Gesehen am 08.05.2020
• Titel Quelle: Enthalten in: European Molecular Biology OrganizationEMBO reports
• Ort Quelle: Heidelberg : EMBO Press, 2000
• Jahr Quelle: 2016
• Band/Heft Quelle: 17(2016), 7, Seite 965-981
• ISSN Quelle: 1469-3178
• DOI: doi:10.15252/embr.201541273
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: ERMES complex
• Sach-SW: IMP
• Sach-SW: Mdm10
• Sach-SW: mitochondria
• Sach-SW: outer membrane
• K10plus-PPN: 169772549X

Abstract

Abstract Mitochondria are separated from the remainder of the eukaryotic cell by the mitochondrial outer membrane (MOM). The MOM plays an important role in different transport processes like lipid trafficking and protein import. In yeast, the ER?mitochondria encounter structure (ERMES) has a central, but poorly defined role in both activities. To understand the functions of the ERMES, we searched for suppressors of the deficiency of one of its components, Mdm10, and identified a novel mitochondrial protein that we named Mdm10 complementing protein 3 (Mcp3). Mcp3 partially rescues a variety of ERMES-related phenotypes. We further demonstrate that Mcp3 is an integral protein of the MOM that follows a unique import pathway. It is recognized initially by the import receptor Tom70 and then crosses the MOM via the translocase of the outer membrane. Mcp3 is next relayed to the TIM23 translocase at the inner membrane, gets processed by the inner membrane peptidase (IMP) and finally integrates into the MOM. Hence, Mcp3 follows a novel biogenesis route where a MOM protein is processed by a peptidase of the inner membrane.