| |  Online-Ressource |
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| Verfasst von: | Katoch, Poonam [VerfasserIn]  |
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| | Gupta, Kinam [VerfasserIn] |
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| | Yennamalli, Ragothaman M. [VerfasserIn] |
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| | Vashistt, Jitendraa [VerfasserIn] |
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| | Bisht, Gopal Singh [VerfasserIn] |
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| | Shrivastava, Rahul [VerfasserIn] |
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| Titel: | Random insertion transposon mutagenesis of Mycobacterium fortuitum identified mutant defective in biofilm formation |
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| Verf.angabe: | Poonam Katoch, Kinam Gupta, Ragothaman M. Yennamalli, Jitendraa Vashistt, Gopal Singh Bisht, Rahul Shrivastava |
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| Jahr: | 2020 |
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| Jahr des Originals: | 2019 |
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| Umfang: | 6 S. |
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| Fussnoten: | Available online 12 November 2019 ; Gesehen am 11.05.2020 |
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| Titel Quelle: | Enthalten in: Biochemical and biophysical research communications |
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| Ort Quelle: | Orlando, Fla. : Academic Press, 1959 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 521(2020), 4, Seite 991-996 |
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| ISSN Quelle: | 1090-2104 |
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| Abstract: | Mycobacterium fortuitum has emerged as a nosocomial infectious agent and biofilm formation attributed for the presence of this bacterium in hospital environment. Transposon random mutagenesis was used to identify membrane-proteins for biofilm formation in M. fortuitum. Ten mutants were shortlisted from a library of 450 mutants for examine their biofilm forming ability. Comparative biofilm ability with respect to wild type M. fortuitum ATCC 6841 showed an altered and delayed biofilm formation in one mutant namely, MT721. Sequence analysis revealed mutation in anthranilate phosphoribosyl transferase (MftrpD), which is associated with tryptophan operon. Functional interaction study of TrpD protein through STRING showed its interaction with chorismate utilizing proteins, majorly involved in synthesis of aromatic amino acid and folic acid, suggesting that biofilm establishment and maintenance requires components of central metabolism. Our study indicates important role of MftrpD in establishment and maintenance of biofilm by M. fortuitum, which may further be explored for drug discovery studies against mycobacterial infections. |
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| DOI: | doi:10.1016/j.bbrc.2019.11.021 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.bbrc.2019.11.021 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X19321412 |
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| | DOI: https://doi.org/10.1016/j.bbrc.2019.11.021 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Anthranilate phosphoribosyltransferase |
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| | Biofilm |
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| | Transposon mutagenesis |
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| K10plus-PPN: | 1698059914 |
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| Verknüpfungen: | → Zeitschrift |
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Random insertion transposon mutagenesis of Mycobacterium fortuitum identified mutant defective in biofilm formation / Katoch, Poonam [VerfasserIn]; 2020 (Online-Ressource)
