Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:García, Sebastián [VerfasserIn]   i
 Swiersy, Anka [VerfasserIn]   i
 Radhakrishnan, Praveen [VerfasserIn]   i
 Branchi, Vittorio [VerfasserIn]   i
 Kanth Nanduri, Lahiri [VerfasserIn]   i
 Győrffy, Balázs [VerfasserIn]   i
 Betzler, Alexander M. [VerfasserIn]   i
 Bork, Ulrich [VerfasserIn]   i
 Kahlert, Christoph [VerfasserIn]   i
 Reißfelder, Christoph [VerfasserIn]   i
 Rahbari, Nuh Nabi [VerfasserIn]   i
 Weitz, Jürgen [VerfasserIn]   i
 Schölch, Sebastian [VerfasserIn]   i
Titel:LDB1 overexpression is a negative prognostic factor in colorectal cancer
Verf.angabe:Sebastián A. García, Anka Swiersy, Praveen Radhakrishnan, Vittorio Branchi, Lahiri Kanth Nanduri, Balázs Győrffy, Alexander M. Betzler, Ulrich Bork, Christoph Kahlert, Christoph Reißfelder, Nuh N. Rahbari, Jürgen Weitz, Sebastian Schölch
E-Jahr:2016
Jahr:October 05, 2016
Umfang:13 S.
Fussnoten:Gesehen am 12.05.2020
Titel Quelle:Enthalten in: OncoTarget
Ort Quelle:[Erscheinungsort nicht ermittelbar] : Impact Journals LLC, 2010
Jahr Quelle:2016
Band/Heft Quelle:7(2016), 51, Seite 84258-84270
ISSN Quelle:1949-2553
Abstract:BACKGROUND: Colorectal cancer (CRC) is the third most common cancer in western countries and is driven by the Wnt signaling pathway. LIM-domain-binding protein 1 (LDB1) interacts with the Wnt signaling pathway and has been connected to malignant diseases. We therefore aimed to evaluate the role of LDB1 in CRC. - RESULTS: Overexpression of LDB1 in CRC is associated with strikingly reduced overall and metastasis free survival in all three independent patient cohorts. The expression of LDB1 positively correlates with genes involved in the Wnt signaling pathway (CTNNB1, AXIN2, MYC and CCND1). Overexpression of LDB1 in CRC cell lines induced Wnt pathway upregulation as well as increased invasivity and proliferation. Upon separate analysis, the role of LDB1 proved to be more prominent in proximal CRC, whereas distal CRC seems to be less influenced by LDB1. - MATERIALS AND METHODS: The expression of LDB1 was measured via RT-qPCR in 59 clinical tumor and normal mucosa samples and correlated to clinical end-points. The role of LDB1 was examined in two additional large patient cohorts from publicly available microarray and RNAseq datasets. Functional characterization was done by lentiviral overexpression of LDB1 in CRC cell lines and TOP/FOP, proliferation and scratch assays. - CONCLUSIONS: LDB1 has a strong role in CRC progression, confirmed in three large, independent patient cohorts. The in vitro data confirm an influence of LDB1 on the Wnt signaling pathway and tumor cell proliferation. LDB1 seems to have a more prominent role in proximal CRC, which confirms the different biology of proximal and distal CRC.
DOI:doi:10.18632/oncotarget.12481
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

DOI: https://doi.org/10.18632/oncotarget.12481
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cell Line, Tumor
 Cell Proliferation
 Cohort Studies
 colorectal cancer
 Colorectal Neoplasms
 Disease Progression
 distal
 DNA-Binding Proteins
 Female
 Gene Expression Profiling
 Gene Expression Regulation, Neoplastic
 HCT116 Cells
 Humans
 Kaplan-Meier Estimate
 ldb1
 LIM Domain Proteins
 Male
 Middle Aged
 Prognosis
 proximal
 Transcription Factors
 wnt signaling
 Wnt Signaling Pathway
K10plus-PPN:1698060440
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68575925   QR-Code
zum Seitenanfang