Synthesis of 5′-NAD-capped RNA

• Verfasst von: Höfer, Katharina [VerfasserIn]
• Verfasst von: Abele, Florian [VerfasserIn]
• Verfasst von: Schlotthauer, Jasmin [VerfasserIn]
• Verfasst von: Jäschke, Andres [VerfasserIn]
• Titel: Synthesis of 5′-NAD-capped RNA
• Verf.angabe: Katharina Höfer, Florian Abele, Jasmin Schlotthauer, Andres Jäschke
• E-Jahr: 2016
• Jahr: March 4, 2016
• Umfang: 4 S.
• Fussnoten: Gesehen am 14.05.2020
• Titel Quelle: Enthalten in: Bioconjugate chemistry
• Ort Quelle: Columbus, Ohio : American Chemical Society, 1990
• Jahr Quelle: 2016
• Band/Heft Quelle: 27(2016), 4, Seite 874-877
• ISSN Quelle: 1520-4812
• DOI: doi:10.1021/acs.bioconjchem.6b00072
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1698276125

Abstract

In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5′-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5′-NAD-capped RNA in high yields and purity under mild conditions. This approach uses unprotected 5′-monophosphate RNA synthesized either chemically or enzymatically, 5′,5′-pyrophosphate bond formation by phosphorimidazolide chemistry, and an enzymatic cleanup step. Thus, 5′-NAD-modified RNA can be synthesized independent of length, structure, and nucleotide sequence.