Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Fellenberg, Jörg [VerfasserIn]   i
 Sähr, Heiner [VerfasserIn]   i
 Kunz, Pierre [VerfasserIn]   i
 Zhao, Zhefu [VerfasserIn]   i
 Liu, Li [VerfasserIn]   i
 Tichy, Diana [VerfasserIn]   i
 Herr, Ingrid [VerfasserIn]   i
Titel:Restoration of miR-127-3p and miR-376a-3p counteracts the neoplastic phenotype of giant cell tumor of bone derived stromal cells by targeting COA1, GLE1 and PDIA6
Verf.angabe:Jörg Fellenberg, Heiner Sähr, Pierre Kunz, Zhefu Zhao, Li Liu, Diana Tichy, Ingrid Herr
Jahr:2016
Jahr des Originals:2015
Umfang:8 S.
Teil:volume:371
 year:2016
 number:1
 pages:134-141
 extent:8
Fussnoten:Available online 30 November 2015 ; Gesehen am 15.05.2020
Titel Quelle:Enthalten in: Cancer letters
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1975
Jahr Quelle:2016
Band/Heft Quelle:371(2016), 1, Seite 134-141
ISSN Quelle:1872-7980
Abstract:Although generally benign, giant cell tumors of bone (GCTB) display an aggressive behavior associated with significant bone destruction and lung metastasis in rare cases. This and the very high recurrence rate observed after surgical resection ranging from 20 to 55% necessitates the development of more effective treatment strategies. To identify valuable therapeutic targets, we screened a previously identified microRNA signature consisting of 23 microRNAs predominantly down-regulated in GCTB. We preselected eight candidate microRNAs and analyzed the impact of their restored expression on the neoplastic phenotype of GCTB stromal cells (GCTSC). A consistent and significant inhibition of cell proliferation, migration, colony formation and spheroid formation could be induced by transfection of primary GCTSC cell lines with miR-127-3p and miR-376a-3p, respectively. Genome wide expression analysis of miR-127-3p and miR-376a-3p transfected cells revealed four novel target genes for each microRNA. Luciferase reporter assays demonstrated direct interactions of miR-127-3p with COA1 and direct interaction of miR-376a-3p with GLE1 and PDIA6, suggesting a pivotal role of these genes in the molecular etiology of GTCB. Interestingly, both microRNAs are located within a chromosomal region frequently silenced in GCTB and many other types of cancers, indicating that these microRNAs and their target genes are valuable therapeutic targets for the treatment of GCTB and possibly other tumor entities.
DOI:doi:10.1016/j.canlet.2015.10.039
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1016/j.canlet.2015.10.039
 Volltext: http://www.sciencedirect.com/science/article/pii/S0304383515007077
 DOI: https://doi.org/10.1016/j.canlet.2015.10.039
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Giant cell tumor of bone
 Mesenchymal stem cell
 MicroRNA
 MiR-127-3p
 MiR-376a-3p
 Neoplastic transformation
K10plus-PPN:1698314434
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68578134   QR-Code
zum Seitenanfang