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| Verfasst von: | Erdel, Fabian [VerfasserIn]  |
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| | Rademacher, Anne [VerfasserIn] |
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| | Vlijm, Rifka [VerfasserIn] |
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| | Tünnermann, Jana [VerfasserIn] |
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| | Frank, Lukas [VerfasserIn] |
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| | Weinmann, Robin [VerfasserIn] |
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| | Schweigert, Elisabeth [VerfasserIn] |
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| | Yserentant, Klaus [VerfasserIn] |
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| | Hummert, Johan [VerfasserIn] |
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| | Bauer, Caroline [VerfasserIn] |
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| | Schumacher, Sabrina [VerfasserIn] |
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| | Al Alwash, Ahmad [VerfasserIn] |
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| | Normand, Christophe [VerfasserIn] |
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| | Herten, Dirk-Peter [VerfasserIn] |
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| | Engelhardt, Johann [VerfasserIn] |
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| | Rippe, Karsten [VerfasserIn] |
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| Titel: | Mouse heterochromatin adopts digital compaction states without showing hallmarks of HP1-driven liquid-liquid phase separation |
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| Verf.angabe: | Fabian Erdel, Anne Rademacher, Rifka Vlijm, Jana Tünnermann, Lukas Frank, Robin Weinmann, Elisabeth Schweigert, Klaus Yserentant, Johan Hummert, Caroline Bauer, Sabrina Schumacher, Ahmad Al Alwash, Christophe Normand, Dirk-Peter Herten, Johann Engelhardt, and Karsten Rippe |
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| E-Jahr: | 2020 |
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| Jahr: | February 25, 2020 |
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| Umfang: | 21 S. |
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| Fussnoten: | Gesehen am 15.05.2020 |
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| Titel Quelle: | Enthalten in: Molecular cell |
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| Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 78(2020), 2, Seite 236-249.e1-e7 |
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| ISSN Quelle: | 1097-4164 |
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| Abstract: | The formation of silenced and condensed heterochromatin foci involves enrichment of heterochromatin protein 1 (HP1). HP1 can bridge chromatin segments and form liquid droplets, but the biophysical principles underlying heterochromatin compartmentalization in the cell nucleus are elusive. Here, we assess mechanistically relevant features of pericentric heterochromatin compaction in mouse fibroblasts. We find that (1) HP1 has only a weak capacity to form liquid droplets in living cells; (2) the size, global accessibility, and compaction of heterochromatin foci are independent of HP1; (3) heterochromatin foci lack a separated liquid HP1 pool; and (4) heterochromatin compaction can toggle between two “digital” states depending on the presence of a strong transcriptional activator. These findings indicate that heterochromatin foci resemble collapsed polymer globules that are percolated with the same nucleoplasmic liquid as the surrounding euchromatin, which has implications for our understanding of chromatin compartmentalization and its functional consequences. |
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| DOI: | doi:10.1016/j.molcel.2020.02.005 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.molcel.2020.02.005 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S1097276520300757 |
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| | DOI: https://doi.org/10.1016/j.molcel.2020.02.005 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | chromatin accessibility |
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| | chromatin compartmentalization |
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| | epigenetic editing |
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| | Heterochromatin protein 1 |
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| | intracellular viscosity |
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| | liquid- liquid phase separation |
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| | nuclear organization |
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| | optodroplets |
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| | polarization-dependent fluorescence correlation spectroscopy |
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| | polymer collapse |
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| K10plus-PPN: | 1698351925 |
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| Verknüpfungen: | → Zeitschrift |
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Mouse heterochromatin adopts digital compaction states without showing hallmarks of HP1-driven liquid-liquid phase separation / Erdel, Fabian [VerfasserIn]; February 25, 2020 (Online-Ressource)
