| Online-Ressource |
Verfasst von: | Korff, Sebastian [VerfasserIn]  |
| Loughran, Patricia [VerfasserIn]  |
| Cai, Changchun [VerfasserIn]  |
| Fan, Jie [VerfasserIn]  |
| Elson, Greg [VerfasserIn]  |
| Shang, Limin [VerfasserIn]  |
| Pires, Susana Salgado [VerfasserIn]  |
| Lee, Yi Shan [VerfasserIn]  |
| Guardado, Jesse [VerfasserIn]  |
| Scott, Melanie [VerfasserIn]  |
| Billiar, Timothy R. [VerfasserIn]  |
Titel: | Tlr2 on bone marrow and non-bbone marrow derived cells regulates inflammation and organ injury in cooperation with Tlr4 during resuscitated hemorrhagic shock |
Verf.angabe: | Sebastian Korff, Patricia Loughran, Changchun Cai, Jie Fan, Greg Elson, Limin Shang, Susana Salgado Pires, Yi Shan Lee, Jesse Guardado, Melanie Scott, and Timothy R. Billiar |
E-Jahr: | 2016 |
Jahr: | 5 May 2016 |
Fussnoten: | Gesehen am 18.05.2020 |
Titel Quelle: | Enthalten in: Shock |
Ort Quelle: | Hagerstown, Md. : Lippincott, Williams & Wilkins, 1994 |
Jahr Quelle: | 2016 |
Band/Heft Quelle: | 46(2016), 5, Seite 519-526$p8 |
ISSN Quelle: | 1540-0514 |
Abstract: | Background: - Although the role of TLR4 in driving inflammation and organ injury after hemorrhagic shock and resuscitation (H/R) is well established, the role of TLR2—another receptor for damage-associated molecular pattern (DAMP) molecules—is not. In this study, we used a combination of TLR2−/− and wild type (WT) mice treated with anti-TLR2 and anti-TLR4 neutralizing monoclonal antibodies (mAb) to discern the contribution of TLR2 relative to TLR4 to the systemic inflammatory response in murine H/R. - Material and Methods: - WT mice, TLR2−/−, and WT mice receiving an anti-TLR2 or an anti-TLR4 mAB (given as a pretreatment) were sacrificed at 6 or 20 h post-H/R. Bone marrow TLR2/WT chimeric mice were created to assess the importance of immune and nonimmune cell-associated TLR2. - Results: - TLR2−/− mice subjected to H/R exhibited significantly less liver damage and lower markers of systemic inflammation only at 20 h. Bone marrow chimeric mice using combinations of TLR2−/− mice and WT mice demonstrated that TLR2 on non-bone marrow derived cells played a dominant role in the differences at 20 h. Interestingly, WT mice treated with anti-TLR2 mAB demonstrated a reduction in organ damage and systemic inflammation at both 6 and 20 h following H/R. A combination of anti-TLR2 mAB and anti-TLR4 mAB showed that both receptors drive IP-10 and KC levels and that there is cooperation for increases in IL-6, MIG, and MCP-1 levels between TLR2 and TLR4. - Conclusion: - These data also support the conclusion that TLR2 and TLR4 act in concert as important receptors in the host immune response to H/R. |
DOI: | doi:10.1097/SHK.0000000000000650 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1097/SHK.0000000000000650 |
| Volltext: https://journals.lww.com/shockjournal/Fulltext/2016/11000/Tlr2_on_Bone_Marrow_and_Non_Bone_Marrow_Derived.8.aspx |
| DOI: https://doi.org/10.1097/SHK.0000000000000650 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1698467664 |
Verknüpfungen: | → Zeitschrift |
Tlr2 on bone marrow and non-bbone marrow derived cells regulates inflammation and organ injury in cooperation with Tlr4 during resuscitated hemorrhagic shock / Korff, Sebastian [VerfasserIn]; 5 May 2016 (Online-Ressource)