Gene expression regulatory networks in Trypanosoma brucei

• Verfasst von: Lueong, Smiths [VerfasserIn]
• Verfasst von: Mercé, Clémentine [VerfasserIn]
• Verfasst von: Fischer, Bernd [VerfasserIn]
• Verfasst von: Hoheisel, Jörg D. [VerfasserIn]
• Verfasst von: Erben, Esteban D. [VerfasserIn]
• Titel: Gene expression regulatory networks in Trypanosoma brucei
• Titelzusatz: insights into the role of the mRNA-binding proteome
• Verf.angabe: Smiths Lueong, Clementine Merce, Bernd Fischer, Jörg D. Hoheisel and Esteban D. Erben
• E-Jahr: 2016
• Jahr: 19 January 2016
• Umfang: 15 S.
• Fussnoten: Gesehen am 19.05.2020
• Titel Quelle: Enthalten in: Molecular microbiology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1987
• Jahr Quelle: 2016
• Band/Heft Quelle: 100(2016), 3, Seite 457-471
• ISSN Quelle: 1365-2958
• DOI: doi:10.1111/mmi.13328
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1698553862

Abstract

Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequences of the protein-mRNA interactions. Here, we provide an overview of the proteins that bind to mRNAs and their functions in the pathogenic bloodstream form of Trypanosoma brucei. We describe the production of a small collection of open-reading frames encoding proteins potentially involved in mRNA metabolism. With this ORFeome collection, we used tethering to screen for proteins that play a role in post-transcriptional control. A yeast two-hybrid screen showed that several of the discovered repressors interact with components of the CAF1/NOT1 deadenylation complex. To identify the RNA-binding proteins, we obtained the mRNA-bound proteome. We identified 155 high-confidence candidates, including many not previously annotated as RNA-binding proteins. Twenty seven of these proteins affected reporter expression in the tethering screen. Our study provides novel insights into the potential trypanosome mRNPs composition, architecture and function.