Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Lueong, Smiths [VerfasserIn]  |
| Mercé, Clémentine [VerfasserIn]  |
| Fischer, Bernd [VerfasserIn]  |
| Hoheisel, Jörg D. [VerfasserIn]  |
| Erben, Esteban D. [VerfasserIn]  |
Titel: | Gene expression regulatory networks in Trypanosoma brucei |
Titelzusatz: | insights into the role of the mRNA-binding proteome |
Verf.angabe: | Smiths Lueong, Clementine Merce, Bernd Fischer, Jörg D. Hoheisel and Esteban D. Erben |
E-Jahr: | 2016 |
Jahr: | 19 January 2016 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 19.05.2020 |
Titel Quelle: | Enthalten in: Molecular microbiology |
Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1987 |
Jahr Quelle: | 2016 |
Band/Heft Quelle: | 100(2016), 3, Seite 457-471 |
ISSN Quelle: | 1365-2958 |
Abstract: | Control of gene expression at the post-transcriptional level is essential in all organisms, and RNA-binding proteins play critical roles from mRNA synthesis to decay. To fully understand this process, it is necessary to identify the complete set of RNA-binding proteins and the functional consequences of the protein-mRNA interactions. Here, we provide an overview of the proteins that bind to mRNAs and their functions in the pathogenic bloodstream form of Trypanosoma brucei. We describe the production of a small collection of open-reading frames encoding proteins potentially involved in mRNA metabolism. With this ORFeome collection, we used tethering to screen for proteins that play a role in post-transcriptional control. A yeast two-hybrid screen showed that several of the discovered repressors interact with components of the CAF1/NOT1 deadenylation complex. To identify the RNA-binding proteins, we obtained the mRNA-bound proteome. We identified 155 high-confidence candidates, including many not previously annotated as RNA-binding proteins. Twenty seven of these proteins affected reporter expression in the tethering screen. Our study provides novel insights into the potential trypanosome mRNPs composition, architecture and function. |
DOI: | doi:10.1111/mmi.13328 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/mmi.13328 |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.13328 |
| DOI: https://doi.org/10.1111/mmi.13328 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1698553862 |
Verknüpfungen: | → Zeitschrift |
Gene expression regulatory networks in Trypanosoma brucei / Lueong, Smiths [VerfasserIn]; 19 January 2016 (Online-Ressource)
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