Status: Bibliographieeintrag
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| Verfasst von: | Best, Marcel [VerfasserIn]  |
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| | Degen, Anna [VerfasserIn] |
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| | Baalmann, Mathis [VerfasserIn] |
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| | Schmidt, Tobias Thomas [VerfasserIn] |
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| | Wombacher, Richard [VerfasserIn] |
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| Titel: | Two-step protein labeling by using lipoic acid ligase with norbornene substrates and subsequent inverse-electron demand Diels-Alder reaction |
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| Verf.angabe: | Marcel Best, Anna Degen, Mathis Baalmann, Tobias T. Schmidt, and Richard Wombacher |
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| E-Jahr: | 2015 |
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| Jahr: | May 5, 2015 |
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| Umfang: | 5 S. |
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| Fussnoten: | Gesehen am 22.05.2020 |
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| Titel Quelle: | Enthalten in: ChemBioChem |
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| Ort Quelle: | Weinheim : Wiley-VCH, 2000 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 16(2015), 8, Seite 1158-1162 |
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| ISSN Quelle: | 1439-7633 |
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| Abstract: | Abstract Inverse-electron-demand Diels?Alder cycloaddition (DAinv) between strained alkenes and tetrazines is a highly bio-orthogonal reaction that has been applied in the specific labeling of biomolecules. In this work we present a two-step labeling protocol for the site-specific labeling of proteins based on attachment of a highly stable norbornene derivative to a specific peptide sequence by using a mutant of the enzyme lipoic acid ligase A (LplAW37V), followed by the covalent attachment of tetrazine-modified fluorophores to the norbornene moiety through the bio-orthogonal DAinv?. We investigated 15 different norbornene derivatives for their selective enzymatic attachment to a 13-residue lipoic acid acceptor peptide (LAP) by using a standardized HPLC protocol. Finally, we used this two-step labeling strategy to label proteins in cell lysates in a site-specific manner and performed cell-surface labeling on living cells. |
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| DOI: | doi:10.1002/cbic.201500042 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/cbic.201500042 |
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| | Volltext: https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201500042 |
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| | DOI: https://doi.org/10.1002/cbic.201500042 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | fluorescent probes |
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| | inverse-electron-demand Diels-Alder cycloaddition |
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| | ligases |
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| | protein labeling |
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| | tetrazines |
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| K10plus-PPN: | 1698696515 |
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| Verknüpfungen: | → Zeitschrift |
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Two-step protein labeling by using lipoic acid ligase with norbornene substrates and subsequent inverse-electron demand Diels-Alder reaction / Best, Marcel [VerfasserIn]; May 5, 2015 (Online-Ressource)
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