Nanoscale alterations of corneocytes indicate skin disease

• Verfasst von: Franz, Jonas [VerfasserIn]
• Verfasst von: Beutel, Moritz [VerfasserIn]
• Verfasst von: Gevers, K. [VerfasserIn]
• Verfasst von: Kramer, A. [VerfasserIn]
• Verfasst von: Thyssen, J. P. [VerfasserIn]
• Verfasst von: Kezic, S. [VerfasserIn]
• Verfasst von: Riethmüller, C. [VerfasserIn]
• Titel: Nanoscale alterations of corneocytes indicate skin disease
• Verf.angabe: J. Franz, M. Beutel, K. Gevers, A. Kramer, J.P. Thyssen, S. Kezic and C. Riethmüller
• Jahr: 2016
• Umfang: 7 S.
• Fussnoten: First published: 22 June 2015 ; Gesehen am 25.05.2020
• Titel Quelle: Enthalten in: Skin research & technology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1995
• Jahr Quelle: 2016
• Band/Heft Quelle: 22(2016), 2, Seite 174-180
• ISSN Quelle: 1600-0846
• DOI: doi:10.1111/srt.12247
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: AFM
• Sach-SW: atopic dermatitis
• Sach-SW: corneocyte
• Sach-SW: nanostructure
• Sach-SW: tape stripping
• K10plus-PPN: 1698724330

Abstract

Background The skin barrier protects the organism against exogenous stressors and simultaneously prevents excessive water loss. While the delicate regulation of skin barrier is not completely understood, morphological and histological evaluation remain key features of clinical investigations. Here, we extended the phenotypic perspective down to the nanoscale. Methods Corneocyte samples were obtained non-invasively by a standard tape stripping procedure from 21 indviduals. Scanning electron (SEM) and atomic force microcopy (AFM) were used to record nanoscale topography. Circular nano-objects were identified and these were quantitated through computer vision. Results Typical dimensions of 273 nm height and 305 nm width. We showed that their density does not correlate to age or pigmentation in healthy subjects, but that they were clearly elevated in corneocytes from patients with atopic dermatitis, a common inflammatory skin condition. Conclusion The presence of these corneocyte-nanostructures might be used as a diagnostic parameter for skin disorders - even in cases below a clinical threshold.