| |  Online-Ressource |
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| Verfasst von: | Kozma, Eszter [VerfasserIn]  |
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| | Nikić, Ivana [VerfasserIn] |
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| | Varga, Balázs R. [VerfasserIn] |
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| | Aramburu, Iker Valle [VerfasserIn] |
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| | Kang, Jun Hee [VerfasserIn] |
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| | Fackler, Oliver Till [VerfasserIn] |
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| | Lemke, Edward A. [VerfasserIn] |
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| | Kele, Péter [VerfasserIn] |
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| Titel: | Hydrophilic trans-cyclooctenylated noncanonical amino acids for fast intracellular protein labeling |
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| Verf.angabe: | Eszter Kozma, Ivana Nikić, Balázs R. Varga, Iker Valle Aramburu, Jun Hee Kang, Oliver T. Fackler, Edward A. Lemke, and Péter Kele |
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| E-Jahr: | 2016 |
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| Jahr: | 25 May 2016 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 25.05.2020 |
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| Titel Quelle: | Enthalten in: ChemBioChem |
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| Ort Quelle: | Weinheim : Wiley-VCH, 2000 |
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| Jahr Quelle: | 2016 |
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| Band/Heft Quelle: | 17(2016), 16, Seite 1518-1524 |
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| ISSN Quelle: | 1439-7633 |
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| Abstract: | Abstract Introduction of bioorthogonal functionalities (e.g., trans-cyclooctene-TCO) into a protein of interest by site-specific genetic encoding of non-canonical amino acids (ncAAs) creates uniquely targetable platforms for fluorescent labeling schemes in combination with tetrazine-functionalized dyes. However, fluorescent labeling of an intracellular protein is usually compromised by high background, arising from the hydrophobicity of ncAAs; this is typically compensated for by hours-long washout to remove excess ncAAs from the cellular interior. To overcome these problems, we designed, synthesized, and tested new, hydrophilic TCO-ncAAs. One derivative, DOTCO-lysine was genetically incorporated into proteins with good yield. The increased hydrophilicity shortened the excess ncAA washout time from hours to minutes, thus permitting rapid labeling and subsequent fluorescence microscopy. |
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| DOI: | doi:10.1002/cbic.201600284 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/cbic.201600284 |
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| | Volltext: https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201600284 |
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| | DOI: https://doi.org/10.1002/cbic.201600284 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | amino acids |
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| | click chemistry |
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| | fluorescence |
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| | hydrophilicity |
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| | protein engineering |
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| K10plus-PPN: | 1698758049 |
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| Verknüpfungen: | → Zeitschrift |
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Hydrophilic trans-cyclooctenylated noncanonical amino acids for fast intracellular protein labeling / Kozma, Eszter [VerfasserIn]; 25 May 2016 (Online-Ressource)
