Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Boon, Kum-Loong [VerfasserIn]  |
| Pearson, Michael David [VerfasserIn]  |
| Koš, Martin [VerfasserIn]  |
Titel: | Self-association of trimethylguanosine synthase Tgs1 is required for efficient snRNA/snoRNA trimethylation and pre-rRNA processing |
Verf.angabe: | Kum-Loong Boon, Michael David Pearson & Martin Koš |
E-Jahr: | 2015 |
Jahr: | 15 June 2015 |
Fussnoten: | Gesehen am 27.05.2020 |
Titel Quelle: | Enthalten in: Scientific reports |
Ort Quelle: | [London] : Macmillan Publishers Limited, part of Springer Nature, 2011 |
Jahr Quelle: | 2015 |
Band/Heft Quelle: | 5(2015) Artikel-Nummer 11282, 12 Seiten |
ISSN Quelle: | 2045-2322 |
Abstract: | Trimethylguanosine Synthase catalyses transfer of two methyl groups to the m7G cap of RNA polymerase II transcribed snRNAs, snoRNAs and telomerase RNA TLC1 to form a 2,2,7-trimethylguanosine cap. While in vitro studies indicate that Tgs1 functions as a monomer and the dimethylation of m7G caps is not a processive reaction, partially methylated sn(o)RNAs are typically not detected in living cells. Here we show that both yeast and human Tgs1p possess a conserved self-association property located at the N-terminus. A disruption of Tgs1 self-association led to a strong reduction of sn(o)RNA trimethylation as well as reduced nucleolar enrichment of Tgs1. Self-association of Tgs1p and its catalytic activity were also prerequisite to bypass the requirement for its accessory factor Swm2p for efficient pre-rRNA processing and snRNA trimethylation. The ability to self-associate might enable Tgs1 to efficiently dimethylate the caps of the targeted RNAs in vivo. |
DOI: | doi:10.1038/srep11282 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1038/srep11282 |
| Volltext: https://www.nature.com/articles/srep11282 |
| DOI: https://doi.org/10.1038/srep11282 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1698883080 |
Verknüpfungen: | → Zeitschrift |
Self-association of trimethylguanosine synthase Tgs1 is required for efficient snRNA/snoRNA trimethylation and pre-rRNA processing / Boon, Kum-Loong [VerfasserIn]; 15 June 2015 (Online-Ressource)
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