| |  Online-Ressource |
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| Verfasst von: | Wiggenhauser, Lucas [VerfasserIn]  |
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| | Qi, Haozhe [VerfasserIn] |
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| | Cramer-Stoll, Sandra [VerfasserIn] |
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| | Metzger, Lena [VerfasserIn] |
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| | Bennewitz, Katrin [VerfasserIn] |
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| | Poschet, Gernot [VerfasserIn] |
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| | Krenning, Guido [VerfasserIn] |
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| | Hillebrands, Jan-Luuk [VerfasserIn] |
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| | Hammes, Hans-Peter [VerfasserIn] |
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| | Kroll, Jens [VerfasserIn] |
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| Titel: | Activation of retinal angiogenesis in hyperglycemic pdx1 -/- zebrafish mutants |
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| Verf.angabe: | Lucas M. Wiggenhauser, Haozhe Qi, Sandra J. Stoll, Lena Metzger, Katrin Bennewitz, Gernot Poschet, Guido Krenning, Jan-Luuk Hillebrands, Hans-Peter Hammes, and Jens Kroll |
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| Jahr: | 2020 |
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| Umfang: | 12 S. |
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| Fussnoten: | Die Zeichen "-/-" sind im Titel hochgestellt ; Gesehen am 28.05.2020 |
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| Titel Quelle: | Enthalten in: Diabetes |
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| Ort Quelle: | Alexandria, Va : Assoc., 1952 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 69(2020), 5, Seite 1020-1031 |
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| ISSN Quelle: | 1939-327X |
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| Abstract: | Progression from the initial vascular response upon hyperglycemia to a proliferative stage with neovacularizations is the hallmark of proliferative diabetic retinopathy. Here, we report on the novel diabetic pdx1(-/-) zebrafish mutant as a model for diabetic retinopathy that lacks the transcription factor pdx1 through CRISPR-Cas9-mediated gene knockout leading to disturbed pancreatic development and hyperglycemia. Larval pdx1(-/-) mutants prominently show vasodilation of blood vessels through increased vascular thickness in the hyaloid network as direct developmental precursor of the adult retinal vasculature in zebrafish. In adult pdx1(-/-) mutants, impaired glucose homeostasis induces increased hyperbranching and hypersprouting with new vessel formation in the retina and aggravation of the vascular alterations from the larval to the adult stage. Both vascular aspects respond to antiangiogenic and antihyperglycemic pharmacological interventions in the larval stage and are accompanied by alterations in the nitric oxide metabolism. Thus, the pdx1(-/-) mutant represents a novel model to study mechanisms of hyperglycemia-induced retinopathy wherein extensive proangiogenic alterations in blood vessel morphology and metabolic alterations underlie the vascular phenotype. |
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| DOI: | doi:10.2337/db19-0873 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.2337/db19-0873 |
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| | DOI: https://doi.org/10.2337/db19-0873 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | agenesis |
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| | diabetic-retinopathy |
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| | endothelial growth-factor |
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| | in-vivo |
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| | insulin-resistance |
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| | metabolism |
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| | model |
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| | nitric-oxide |
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| | pancreas |
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| | vessels |
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| K10plus-PPN: | 1698947925 |
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| Verknüpfungen: | → Zeitschrift |
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Activation of retinal angiogenesis in hyperglycemic pdx1 -/- zebrafish mutants / Wiggenhauser, Lucas [VerfasserIn]; 2020 (Online-Ressource)
