Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin

• Verfasst von: Maier, Timo [VerfasserIn]
• Verfasst von: Haraszti, Tamás [VerfasserIn]
• Titel: Reversibility and viscoelastic properties of micropillar supported and oriented magnesium bundled F-actin
• Verf.angabe: Timo Maier, Tamás Haraszti
• E-Jahr: 2015
• Jahr: August 31, 2015
• Fussnoten: Gesehen am 05.06.2020
• Titel Quelle: Enthalten in: PLOS ONE
• Ort Quelle: San Francisco, California, US : PLOS, 2006
• Jahr Quelle: 2015
• Band/Heft Quelle: 10(2015,8) Artikel-Nummer e0136432, 17 Seiten
• ISSN Quelle: 1932-6203
• DOI: doi:10.1371/journal.pone.0136432
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Actin filaments
• Sach-SW: Actin polymerization
• Sach-SW: Cations
• Sach-SW: Cross-linking
• Sach-SW: Fluorescence imaging
• Sach-SW: Ions
• Sach-SW: Magnesium
• Sach-SW: Polymerization
• K10plus-PPN: 169995836X

Abstract

Filamentous actin is one of the most important cytoskeletal elements. Not only is it responsible for the elastic properties of many cell types, but it also plays a vital role in cellular adhesion and motility. Understanding the bundling kinetics of actin filaments is important in the formation of various cytoskeletal structures, such as filopodia and stress fibers. Utilizing a unique pillar-structured microfluidic device, we investigated the time dependence of bundling kinetics of pillar supported free-standing actin filaments. Microparticles attached to the filaments allowed the measurement of thermal motion, and we found that bundling takes place at lower concentrations than previously found in 3-dimensional actin gels, i.e. actin filaments formed bundles in the presence of 5-12 mM of magnesium chloride in a time-dependent manner. The filaments also displayed long term stability for up to hours after removing the magnesium ions from the buffer, which suggests that there is an extensive hysteresis between cation induced crosslinking and decrosslinking.