Differentiation of ICOS+ and ICOS- recent thymic emigrant regulatory T cells (RTE Tregs) during normal pregnancy, pre-eclampsia and HELLP syndrome

• Verfasst von: Wagner, Miriam [VerfasserIn]
• Verfasst von: Jöst, Maria [VerfasserIn]
• Verfasst von: Spratte, Julia [VerfasserIn]
• Verfasst von: Schaier, Matthias [VerfasserIn]
• Verfasst von: Mahnke, Karsten [VerfasserIn]
• Verfasst von: Meuer, Stefan [VerfasserIn]
• Verfasst von: Zeier, Martin [VerfasserIn]
• Verfasst von: Steinborn-Kröhl, Andrea [VerfasserIn]
• Titel: Differentiation of ICOS+ and ICOS- recent thymic emigrant regulatory T cells (RTE Tregs) during normal pregnancy, pre-eclampsia and HELLP syndrome
• Verf.angabe: M. I. Wagner, M. Jöst, J. Spratte, M. Schaier, K. Mahnke, S. Meuer, M. Zeier and A. Steinborn
• E-Jahr: 2016
• Jahr: [2016]
• Jahr des Originals: 2015
• Umfang: 14 S.
• Fussnoten: First published: 18 August 2015 ; Das Plus- und Minuszeichen im Titel ist hochgestellt, regs ist tiefgestellt ; Gesehen am 05.06.2020
• Titel Quelle: Enthalten in: Clinical & experimental immunology
• Ort Quelle: Oxford : Wiley-Blackwell, 1966
• Jahr Quelle: 2016
• Band/Heft Quelle: 183(2016), 1, Seite 129-142
• ISSN Quelle: 1365-2249
• DOI: doi:10.1111/cei.12693
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: HELLP syndrome
• Sach-SW: ICOS+ and ICOS− Tregs
• Sach-SW: immune suppression
• Sach-SW: pre-eclampsia
• Sach-SW: pregnancy
• K10plus-PPN: 1699963398

Abstract

Two different subsets of naturally occurring regulatory T cells (nTregs), defined by their expression of the inducible co-stimulatory (ICOS) molecule, are produced by the human thymus. To examine the differentiation of ICOS+ and ICOS−CD45RA+CD31+ recent thymic emigrant (RTE) Tregs during normal pregnancy and in the presence of pre-eclampsia or haemolysis elevated liver enzymes low platelet (HELLP)-syndrome, we used six-colour flow cytometric analysis to determine the changes in the composition of the ICOS+ and ICOS− Treg pools with CD45RA+CD31+ RTE Tregs, CD45RA+CD31− mature naive (MN) Tregs, CD45RA−CD31+ and CD45RA−CD31− memory Tregs. With the beginning of pregnancy until term, we observed a strong differentiation of both ICOS+ and ICOS−CD45RA+CD31+ RTE, but not CD45RA+CD31− MN Tregs, into CD45RA−CD31− memory Tregs. At the end of pregnancy, the onset of spontaneous term labour was associated with a significant breakdown of ICOS+CD45RA−CD31− memory Tregs. However, in the presence of pre-eclampsia, there was a significantly increased differentiation of ICOS+ and ICOS−CD45RA+CD31+ RTE Tregs into CD45RA−CD31+ memory Tregs, wherein the lacking differentiation into CD45RA−CD31− memory Tregs was partially replaced by the increased differentiation of ICOS+ and ICOS−CD45RA+CD31− MN Tregs into CD45RA−CD31− memory Tregs. In patients with HELLP syndrome, this alternatively increased differentiation of CD45RA−CD31− MN Tregs seemed to be exaggerated, and presumably restored the suppressive activity of magnetically isolated ICOS+ and ICOS− Tregs, which were shown to be significantly less suppressive in pre-eclampsia patients, but not in HELLP syndrome patients. Hence, our findings propose that the regular differentiation of both ICOS+ and ICOS−CD45RA+CD31+ RTE Tregs ensures a healthy pregnancy course, while their disturbed differentiation is associated with the occurrence of pre-eclampsia and HELLP syndrome.