CD56-positive lymphocyte infiltration in relation to human papillomavirus association and prognostic significance in oropharyngeal squamous cell carcinoma

• Verfasst von: Wagner, Steffen [VerfasserIn]
• Verfasst von: Wittekindt, Claus [VerfasserIn]
• Verfasst von: Reuschenbach, Miriam [VerfasserIn]
• Verfasst von: Hennig, Ben [VerfasserIn]
• Verfasst von: Thevarajah, Mauran [VerfasserIn]
• Verfasst von: Würdemann, Nora [VerfasserIn]
• Verfasst von: Prigge, Elena-Sophie [VerfasserIn]
• Verfasst von: Knebel Doeberitz, Magnus von [VerfasserIn]
• Verfasst von: Dreyer, Thomas [VerfasserIn]
• Verfasst von: Gattenlöhner, Stefan [VerfasserIn]
• Verfasst von: Klussmann, Jens Peter [VerfasserIn]
• Titel: CD56-positive lymphocyte infiltration in relation to human papillomavirus association and prognostic significance in oropharyngeal squamous cell carcinoma
• Verf.angabe: Steffen Wagner, Claus Wittekindt, Miriam Reuschenbach, Ben Hennig, Mauran Thevarajah, Nora Würdemann, Elena-Sophie Prigge, Magnus von Knebel Doeberitz, Thomas Dreyer, Stefan Gattenlöhner and Jens Peter Klussmann
• E-Jahr: 2016
• Jahr: [2016]
• Jahr des Originals: 2015
• Umfang: 11 S.
• Illustrationen: Illustrationen
• Fussnoten: Online 13 Dec 2015 ; Gesehen am 05.06.2020
• Titel Quelle: Enthalten in: International journal of cancer
• Ort Quelle: Bognor Regis : Wiley-Liss, 1966
• Jahr Quelle: 2016
• Band/Heft Quelle: 138(2016), 9, Seite 2263-2273
• ISSN Quelle: 1097-0215
• DOI: doi:10.1002/ijc.29962
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: CD56
• Sach-SW: head and neck
• Sach-SW: HPV
• Sach-SW: natural killer cell
• Sach-SW: orpharyngeal squamous cell carcinoma
• K10plus-PPN: 1699965811

Abstract

Human papillomavirus (HPV)-related squamous cell carcinoma of the oropharynx (OSCC) are clinical and biological distinct from their HPV-unrelated counterparts. Patients with HPV-related OSCC display improved prognosis and therefore we investigated possible immune cell infiltrations associated with this tumor phenotype. We retrospectively analyzed a randomly selected cohort of 140 OSCC for presence of immune cells and HPV by immunohistochemistry and PCR followed by bead-based hybridization (Luminex technology). HPV prevalence was 24.3% as determined by positive staining of p16INK4a and detection of high risk HPV-DNA. We found significantly higher numbers of CD56 positive (CD56+) cells in tumor and surrounding microenvironment in HPV-associated compared to HPV-negative OSCC (t-test: p = 0.004 and p = 0.002). For the entire cohort presence of CD56+ cells was associated with increased overall survival independent from HPV (Kaplan-Meier: p = 0.002; Cox regression: p = 0.042). Presence of CD56+ cells also correlated with a better outcome in HPV-negative and especially in HPV-negative OSCC with alcohol consumption ≤2 standard drinks per day (Kaplan-Meier: p = 0.05 and p = 0.003). Immunofluorescence localization of granular Granzyme B (GZMB) within CD56+ cells and coexpression of CD16 and CD56 suggests that detected CD56+ cells mainly represent cytotoxic Natural Killer (NK) cells. The fraction of potentially cytotoxic NK cells was significantly higher in HPV-associated compared to HPV-negative OSCC (Mann-Whitney-U-Test: p = 0.011). The elevated abundance and activity of cytotoxic NK cells in OSCC with HPV driven carcinogenesis might contribute to favorable outcome in HPV-related OSCC.