Evidence for the anti-inflammatory activity of Bupleurum marginatum (Apiaceae) extracts using in vitro and in vivo experiments supported by virtual screening

• Verfasst von: Ashour, Mohamed L. [VerfasserIn]
• Verfasst von: Youssef, Fadia S. [VerfasserIn]
• Verfasst von: Gad, Haidy A. [VerfasserIn]
• Verfasst von: Readi, Mahmoud Zaki el [VerfasserIn]
• Verfasst von: Bouzabata, Amel [VerfasserIn]
• Verfasst von: Abuzeid, Riham M. [VerfasserIn]
• Verfasst von: Sobeh, Mansour [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Evidence for the anti-inflammatory activity of Bupleurum marginatum (Apiaceae) extracts using in vitro and in vivo experiments supported by virtual screening
• Verf.angabe: Mohamed L. Ashour, Fadia S. Youssef, Haidy A. Gad, Mahmoud Z. El‐Readi, Amel Bouzabata, Riham M. Abuzeid, Mansour Sobeh, and Michael Wink
• E-Jahr: 2018
• Jahr: 03 April 2018
• Umfang: 12 S.
• Fussnoten: Gesehen am 08.06.2020
• Titel Quelle: Enthalten in: Journal of pharmacy and pharmacology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1949
• Jahr Quelle: 2018
• Band/Heft Quelle: 70(2018), 7, Seite 952-963
• ISSN Quelle: 2042-7158
• DOI: doi:10.1111/jphp.12904
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 5-lipoxygenase
• Sach-SW: anti-inflammatory activity
• Sach-SW: Bupleurum
• Sach-SW: cyclooxygenase II
• Sach-SW: paw oedeme
• Sach-SW: virtual screening
• K10plus-PPN: 170016645X

Abstract

Objectives To explore the potential anti-inflammatory activity of Bupleurum marginatum extracts using in vitro and in vivo studies supported by virtual screening. Methods Antioxidant activity was assessed using the DPPH˙ and inhibition of 2-deoxyribose degradation assays. Anti-inflammatory activity was determined in vitro by measuring the suppression of prostaglandin E2 release (PGE2) in pancreatic cancer cells (MIA-PaCa-2) and the inhibition of 5-lipoxygenase whereas the rat paw oedema was used in vivo. The major constituents were docked in 5-lipoxygenase and cyclooxygenase-II active sites. Key findings Methanol and dichloromethane (DCM) extracts showed IC50 of 46.99 and 162.99 μg/ml in the DPPH˙, 1.52 and 2.12 μg/ml in inhibition of 2-deoxyribose degradation assays, respectively. They reduced PGE2 release by 41.33 and 52.85% at 25 μg/ml and inhibited 5-lipoxygenase with IC50 of 45.28 and 25.92 μg/ml, respectively. 50 and 70% reduction in the diameter of the carrageenan-induced rat paws with methanol and DCM extracts, respectively, with a marked decline in the inflammation score was observed. Rutin, a predominating compound, showed a strong interaction with the key amino acids in 5-LOX active site with interaction energy of −74.59 kcal/mol. Conclusions Our study provides evidence for an interesting anti-inflammatory activity of B. marginatum aerial parts offering a natural anti-inflammatory agent.