Understanding intramembrane proteolysis

• Verfasst von: Langosch, Dieter [VerfasserIn]
• Verfasst von: Scharnagl, C. [VerfasserIn]
• Verfasst von: Steiner, H. [VerfasserIn]
• Verfasst von: Lemberg, Marius [VerfasserIn]
• Titel: Understanding intramembrane proteolysis
• Titelzusatz: from protein dynamics to reaction kinetics
• Verf.angabe: D. Langosch, C. Scharnagl, H. Steiner, and M.K. Lemberg
• E-Jahr: 2015
• Jahr: 1 May 2015
• Umfang: 10 S.
• Fussnoten: Gesehen am 09.06.2020
• Titel Quelle: Enthalten in: Trends in biochemical sciences
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1976
• Jahr Quelle: 2015
• Band/Heft Quelle: 40(2015), 6, Seite 318-327
• ISSN Quelle: 1362-4326
• DOI: doi:10.1016/j.tibs.2015.04.001
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: conformational dynamics
• Sach-SW: intramembrane proteolysis/protease
• Sach-SW: presenilin
• Sach-SW: rhomboid
• Sach-SW: transmembrane helix
• Sach-SW: γ-secretase
• K10plus-PPN: 1700241796

Abstract

Intramembrane proteolysis - cleavage of proteins within the plane of a membrane - is a widespread phenomenon that can contribute to the functional activation of substrates and is involved in several diseases. Although different families of intramembrane proteases have been discovered and characterized, we currently do not know how these enzymes discriminate between substrates and non-substrates, how site-specific cleavage is achieved, or which factors determine the rate of proteolysis. Focusing on γ-secretase and rhomboid proteases, we argue that answers to these questions may emerge from connecting experimental readouts, such as reaction kinetics and the determination of cleavage sites, to the structures and the conformational dynamics of substrates and enzymes.