Combinatorial control of light induced chromatin remodeling and gene activation in neurospora

• Verfasst von: Sancar, Cigdem [VerfasserIn]
• Verfasst von: Ha, Nati [VerfasserIn]
• Verfasst von: Yılmaz, Rüstem [VerfasserIn]
• Verfasst von: Tesorero, Rafael [VerfasserIn]
• Verfasst von: Fischer, Tamás [VerfasserIn]
• Verfasst von: Brunner, Michael [VerfasserIn]
• Verfasst von: Sancar, Gencer [VerfasserIn]
• Titel: Combinatorial control of light induced chromatin remodeling and gene activation in neurospora
• Verf.angabe: Cigdem Sancar, Nati Ha, Rüstem Yilmaz, Rafael Tesorero, Tamas Fisher, Michael Brunner, Gencer Sancar
• E-Jahr: 2015
• Jahr: March 30, 2015
• Umfang: 26 S.
• Fussnoten: Gesehen am 10.06.2020
• Titel Quelle: Enthalten in: Public Library of SciencePLoS Genetics
• Ort Quelle: San Francisco, Calif. : Public Library of Science, 2005
• Jahr Quelle: 2015
• Band/Heft Quelle: 11(2015,3) Artikel-Nummer 1005105, 26 Seiten
• ISSN Quelle: 1553-7404
• DOI: doi:10.1371/journal.pgen.1005105
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Binding analysis
• Sach-SW: DNA transcription
• Sach-SW: Gene expression
• Sach-SW: Gene regulation
• Sach-SW: Neurospora
• Sach-SW: Nucleosome mapping
• Sach-SW: Nucleosomes
• Sach-SW: Sequence motif analysis
• K10plus-PPN: 1700331981

Abstract

Light is an important environmental cue that affects physiology and development of Neurospora crassa. The light-sensing transcription factor (TF) WCC, which consists of the GATA-family TFs WC1 and WC2, is required for light-dependent transcription. SUB1, another GATA-family TF, is not a photoreceptor but has also been implicated in light-inducible gene expression. To assess regulation and organization of the network of light-inducible genes, we analyzed the roles of WCC and SUB1 in light-induced transcription and nucleosome remodeling. We show that SUB1 co-regulates a fraction of light-inducible genes together with the WCC. WCC induces nucleosome eviction at its binding sites. Chromatin remodeling is facilitated by SUB1 but SUB1 cannot activate light-inducible genes in the absence of WCC. We identified FF7, a TF with a putative O-acetyl transferase domain, as an interaction partner of SUB1 and show their cooperation in regulation of a fraction of light-inducible and a much larger number of non light-inducible genes. Our data suggest that WCC acts as a general switch for light-induced chromatin remodeling and gene expression. SUB1 and FF7 synergistically determine the extent of light-induction of target genes in common with WCC but have in addition a role in transcription regulation beyond light-induced gene expression.