Leukocyte responses to immobilized patterns of CXCL8

• Verfasst von: Girrbach, Maria [VerfasserIn]
• Verfasst von: Rink, Ina [VerfasserIn]
• Verfasst von: Ladnorg, Tatjana [VerfasserIn]
• Verfasst von: Azucena, Carlos [VerfasserIn]
• Verfasst von: Heißler, Stefan [VerfasserIn]
• Verfasst von: Haraszti, Tamás [VerfasserIn]
• Verfasst von: Schepers, Ute [VerfasserIn]
• Verfasst von: Schmitz, Katja [VerfasserIn]
• Titel: Leukocyte responses to immobilized patterns of CXCL8
• Verf.angabe: Maria Girrbach, Ina Rink, Tatjana Ladnorg, Carlos Azucena, Stefan Heißler, Tamás Haraszti, Ute Schepers, Katja Schmitz
• E-Jahr: 2016
• Jahr: 2 March 2016
• Umfang: 7 S.
• Fussnoten: Gesehen am 12.06.2020
• Titel Quelle: Enthalten in: Colloids and surfaces / B
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1993
• Jahr Quelle: 2016
• Band/Heft Quelle: 142(2016), Seite 385-391
• ISSN Quelle: 1873-4367
• DOI: doi:10.1016/j.colsurfb.2016.03.004
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Biofunctional surfaces
• Sach-SW: Chemokines
• Sach-SW: Microcontact printing
• Sach-SW: Protein patterns
• K10plus-PPN: 1700499564

Abstract

The attachment of neutrophils to the endothelial surface and their migration towards the site of inflammation following chemokine gradients play an essential role in the innate immune response. Chemokines adhere to glycosaminoglycans on the endothelial surface to be detected by leukocytes and trigger their movement along surface- bound gradients in a process called haptotaxis. In assays to systematically study the response of leukocytes to surface-bound compounds both the spatial arrangement of the compound as well as the mode of immobilization need to be controlled. In this study microcontact printing was employed to create patterns of hydrophobic or functionalized thiols on gold-coated glass slides and CXCL8 was immobilized on the thiol coated areas using three different strategies. Human neutrophils adhered to the CXCL8-coated lines but not to the PEG-coated background. We could show that more cells adhered to CXCL8 adsorbed to hydrophobic octadecanethiol than on CXCL8 covalently bound to amino undecanethiol or CXCL8 specifically bound to immobilized heparin on aminothiol. Likewise general cell activity such as lamellipodia formation and random migration were most pronounced for CXCL8 adsorbed on a hydrophobic surface which may be attributed to the larger amounts of protein immobilized on this type of surface.