Unconventional apoptosis of polymorphonuclear neutrophils (PMN)

• Verfasst von: Franz, Sandra [VerfasserIn]
• Verfasst von: Muñoz Becerra, Luis Enrique [VerfasserIn]
• Verfasst von: Heyder, Petra [VerfasserIn]
• Verfasst von: Herrmann, Martin [VerfasserIn]
• Verfasst von: Schiller, Martin [VerfasserIn]
• Titel: Unconventional apoptosis of polymorphonuclear neutrophils (PMN)
• Titelzusatz: staurosporine delays exposure of phosphatidylserine and prevents phagocytosis by M[phi]-2 macrophages of PMN
• Verf.angabe: S. Franz, L.E. Muñoz, P. Heyder, M. Herrmann and M. Schiller
• Jahr: 2015
• Jahr des Originals: 2014
• Umfang: 10 S.
• Fussnoten: Im Titel ist "phi" als griechischer Buchstabe dargestellt ; First published: 03 July 2014 ; Gesehen am 12.06.2020
• Titel Quelle: Enthalten in: Clinical & experimental immunology
• Ort Quelle: Oxford : Wiley-Blackwell, 1966
• Jahr Quelle: 2015
• Band/Heft Quelle: 179(2015), 1, Seite 75-84
• ISSN Quelle: 1365-2249
• DOI: doi:10.1111/cei.12412
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: apoptosis
• Sach-SW: clearance
• Sach-SW: opsonization
• Sach-SW: PMN
• Sach-SW: staurosporine
• K10plus-PPN: 1700528386

Abstract

Apoptosis of polymorphonuclear neutrophils (PMN) and subsequent ‘silent’ removal represents an important check-point for the resolution of inflammation. Failure in PMN clearance resulting in secondary necrosis-driven tissue damage has been implicated in conditions of chronic inflammation and autoimmunity. Apoptotic PMN undergo profound biophysical changes that warrant their efficient recognition and uptake by phagocytes before fading to secondary necrosis. In this study, we demonstrate that staurosporine (STS), a non-selective but potent inhibitor of cyclin-dependent kinase and protein kinase C, exerts a drastic impact on PMN apoptosis. PMN treated with STS underwent an unconventional form of cell death characterized by a delayed exposure of aminophospholipids, including phosphatidylserine (PS) and phosphatidylethanolamine and an increased exposure of neo-glycans. STS caused an impaired cellular fragmentation and accelerated DNA fragmentation. Phagocytosis of STS-treated PMN lacking PS on their surfaces was decreased significantly, which highlights the importance of PS for the clearance of apoptotic PMN. Specific opsonization with immune complexes completely restored phagocytosis of STS-treated PMN, demonstrating the efficiency of back-up clearance pathways in the absence of PS exposure.