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| Verfasst von: | Dokić, Ivana [VerfasserIn]  |
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| | Niklas, Martin Anthony [VerfasserIn] |
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| | Zimmermann, Ferdinand [VerfasserIn] |
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| | Mairani, Andrea [VerfasserIn] |
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| | Seidel, Philipp [VerfasserIn] |
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| | Krunic, Damir [VerfasserIn] |
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| | Jäkel, Oliver [VerfasserIn] |
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| | Debus, Jürgen [VerfasserIn] |
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| | Greilich, Steffen [VerfasserIn] |
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| | Abdollahi, Amir [VerfasserIn] |
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| Titel: | Correlation of particle traversals with clonogenic survival using cell-fluorescent ion track hybrid detector |
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| Verf.angabe: | Ivana Dokic, Martin Niklas, Ferdinand Zimmermann, Andrea Mairani, Philipp Seidel, Damir Krunic, Oliver Jäkel, Jürgen Debus, Steffen Greilich and Amir Abdollahi |
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| E-Jahr: | 2015 |
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| Jahr: | 07 December 2015 |
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| Umfang: | 7 S. |
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| Teil: | volume:5 |
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| | year:2015 |
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| | extent:7 |
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| Fussnoten: | Gesehen am 15.06.2020 |
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| Titel Quelle: | Enthalten in: Frontiers in oncology |
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| Ort Quelle: | Lausanne : Frontiers Media, 2011 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 5(2015) Artikel-Nummer 275, 7 Seiten |
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| ISSN Quelle: | 2234-943X |
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| Abstract: | Development of novel approaches linking the physical characteristics of particles with biological responses are of high relevance for the field of particle therapy. In radiobiology, the clonogenic survival of cells is considered the gold standard assay for assessment of cellular sensitivity to ionizing radiation. Towards further development of next generation biodosimeters in particle therapy, cell-fluorescent ion track hybrid detector (Cell-FIT-HD) was recently engineered by our group and successfully employed to study physical particle track information in correlation with irradiation- induced DNA damage in cell nuclei. In this work, we investigated the feasibility of Cell-FIT-HD as a tool to study the effects of clinical beams on cellular clonogenic survival. Tumor cells were grown on the FNTD as cell culture, mimicking the standard procedures for clonogenic assay. Cell-FIT-HD was used to detect the spatial distribution of particle tracks within colony-initiating cells. The physical data were associated to radiation induced foci as surrogates for DNA double strand breakages (DSB), the hallmark of radiation ‐induced cell lethality. Long‐term cell fate was monitored to determine the ability of cells to form colonies. We report the first successful detection of particle traversal within colony-initiating cells at subcellular resolution using Cell-FIT-HD. |
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| DOI: | doi:10.3389/fonc.2015.00275 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3389/fonc.2015.00275 |
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| | Volltext: https://www.frontiersin.org/articles/10.3389/fonc.2015.00275/full |
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| | DOI: https://doi.org/10.3389/fonc.2015.00275 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 53BP1 |
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| | Carbon ion irradiation |
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| | Clonogenic Survival |
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| | DNA damage foci |
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| | Fluorescent nuclear track detector |
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| K10plus-PPN: | 1700560832 |
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| Verknüpfungen: | → Zeitschrift |
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Correlation of particle traversals with clonogenic survival using cell-fluorescent ion track hybrid detector / Dokić, Ivana [VerfasserIn]; 07 December 2015 (Online-Ressource)
