Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Schwörer, Felicitas [VerfasserIn]   i
 Trapp, Marcus [VerfasserIn]   i
 Ballauff, Matthias [VerfasserIn]   i
 Dahint, Reiner [VerfasserIn]   i
 Steitz, Roland [VerfasserIn]   i
Titel:Surface-active lipid linings under shear load
Titelzusatz:a combined in-situ neutron reflectivity and ATR-FTIR study
Verf.angabe:Felicitas Schwörer, Marcus Trapp, Matthias Ballauff, Reiner Dahint, and Roland Steitz
E-Jahr:2015
Jahr:September 21, 2015
Umfang:10 S.
Fussnoten:Gesehen am 15.06.2020
Titel Quelle:Enthalten in: Langmuir
Ort Quelle:Washington, DC : ACS Publ., 1985
Jahr Quelle:2015
Band/Heft Quelle:31(2015), 42, Seite 11539-11548
ISSN Quelle:1520-5827
Abstract:We study shear effects in solid-supported lipid membrane stacks by simultaneous combined in-situ neutron reflectivity (NR) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). The stacks mimic the terminal surface-active phospholipid (SAPL) coatings on cartilage in mammalian joints. Piles of 11 bilayer membranes of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) are immobilized at the interface of the solid silicon support and the liquid D2O backing phase. We replace the natural hyaluronic acid (HA) component of synovial fluid by a synthetic substitute, namely, poly(allylamine hydrochloride) (PAH), at identical concentration. We find the oligolamellar DMPC bilayer films strongly interacting with PAH resulting in a drastic increase of the membranes d spacing (by a factor of ∼5). Onset of shear causes a buckling-like deformation of the DMPC bilayers perpendicular to the applied shear field. With increasing shear rate we observe substantially enhanced water fractions in the membrane slabs which we attribute to increasing fragmentation caused by Kelvin-Helmholtz-like instabilities parallel to the applied shear field. Both effects are in line with recent theoretical predictions on shear-induced instabilities of lipid bilayer membranes in water (Hanasaki, I.; Walther, J. H.; Kawano, S.; Koumoutsakos, P. Phys. Rev. E 2010, 82, 051602). With the applied shear the interfacial lipid linings transform from their gel state Pβ′ to their fluid state Lα. Although in chain-molten state with reduced bending rigidity the lipid layers do not detach from their solid support. We hold steric bridging of the fragmented lipid bilayer membranes by PAH molecules responsible for the unexpected mechanical stability of the DMPC linings.
DOI:doi:10.1021/acs.langmuir.5b01678
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1021/acs.langmuir.5b01678
 DOI: https://doi.org/10.1021/acs.langmuir.5b01678
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1700587927
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68586790   QR-Code
zum Seitenanfang