Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells

• Verfasst von: Sauter, Alexander [VerfasserIn]
• Verfasst von: McDuffie, Yvonne [VerfasserIn]
• Verfasst von: Böhm, Heike [VerfasserIn]
• Verfasst von: Martinez, A. [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Verfasst von: Otto, Silke [VerfasserIn]
• Titel: Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells
• Verf.angabe: A. Sauter, Y. Mc Duffie, H. Boehm, A. Martinez, J.P. Spatz & S. Appel
• Jahr: 2015
• Jahr des Originals: 2014
• Umfang: 10 S.
• Fussnoten: First published 07 November 2014 ; Gesehen am 16.06.2020
• Titel Quelle: Enthalten in: Scandinavian journal of immunology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1972
• Jahr Quelle: 2015
• Band/Heft Quelle: 81(2015), 1, Seite 56-65
• ISSN Quelle: 1365-3083
• DOI: doi:10.1111/sji.12246
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1700650505

Abstract

Ex vivo-generated human dendritic cells (DC) are most commonly generated from monocytes using standard cell culture dishes. To elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with four different mainly non-adherent surfaces. Untouched monocytes were cultured for 3 days in the presence of IL-4 and GM-CSF. Time-lapse videos were recorded, and the phenotype of the cells was analysed by flow cytometry. The cytokine profiles were analysed using a 25-plex cytokine assay. The use of non-adherent surfaces led to a significant reduction in expression of CD14 and CD38, and a significant increase in expression of CD86 compared to standard culture dishes. Expression levels of DC-SIGN and PD-L2 were reduced significantly on cells cultured on non-adherent surfaces. The cytokine production was independent on the surface used. The surface-mediated priming should therefore be considered when aiming to induce specific immune responses. This is especially important with regard to DC-based immunotherapy, where an adjustment of the surface during the DC generation process might have highly beneficial effects.