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Verfasst von:Sauter, Alexander [VerfasserIn]   i
 McDuffie, Yvonne [VerfasserIn]   i
 Böhm, Heike [VerfasserIn]   i
 Martinez, A. [VerfasserIn]   i
 Spatz, Joachim P. [VerfasserIn]   i
 Otto, Silke [VerfasserIn]   i
Titel:Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells
Verf.angabe:A. Sauter, Y. Mc Duffie, H. Boehm, A. Martinez, J.P. Spatz & S. Appel
Jahr:2015
Jahr des Originals:2014
Umfang:10 S.
Fussnoten:First published 07 November 2014 ; Gesehen am 16.06.2020
Titel Quelle:Enthalten in: Scandinavian journal of immunology
Ort Quelle:Oxford [u.a.] : Wiley-Blackwell, 1972
Jahr Quelle:2015
Band/Heft Quelle:81(2015), 1, Seite 56-65
ISSN Quelle:1365-3083
Abstract:Ex vivo-generated human dendritic cells (DC) are most commonly generated from monocytes using standard cell culture dishes. To elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with four different mainly non-adherent surfaces. Untouched monocytes were cultured for 3 days in the presence of IL-4 and GM-CSF. Time-lapse videos were recorded, and the phenotype of the cells was analysed by flow cytometry. The cytokine profiles were analysed using a 25-plex cytokine assay. The use of non-adherent surfaces led to a significant reduction in expression of CD14 and CD38, and a significant increase in expression of CD86 compared to standard culture dishes. Expression levels of DC-SIGN and PD-L2 were reduced significantly on cells cultured on non-adherent surfaces. The cytokine production was independent on the surface used. The surface-mediated priming should therefore be considered when aiming to induce specific immune responses. This is especially important with regard to DC-based immunotherapy, where an adjustment of the surface during the DC generation process might have highly beneficial effects.
DOI:doi:10.1111/sji.12246
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1111/sji.12246
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/sji.12246
 DOI: https://doi.org/10.1111/sji.12246
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1700650505
Verknüpfungen:→ Zeitschrift

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