Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Sauter, Alexander [VerfasserIn]  |
| McDuffie, Yvonne [VerfasserIn]  |
| Böhm, Heike [VerfasserIn]  |
| Martinez, A. [VerfasserIn]  |
| Spatz, Joachim P. [VerfasserIn]  |
| Otto, Silke [VerfasserIn]  |
Titel: | Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells |
Verf.angabe: | A. Sauter, Y. Mc Duffie, H. Boehm, A. Martinez, J.P. Spatz & S. Appel |
Jahr: | 2015 |
Jahr des Originals: | 2014 |
Umfang: | 10 S. |
Fussnoten: | First published 07 November 2014 ; Gesehen am 16.06.2020 |
Titel Quelle: | Enthalten in: Scandinavian journal of immunology |
Ort Quelle: | Oxford [u.a.] : Wiley-Blackwell, 1972 |
Jahr Quelle: | 2015 |
Band/Heft Quelle: | 81(2015), 1, Seite 56-65 |
ISSN Quelle: | 1365-3083 |
Abstract: | Ex vivo-generated human dendritic cells (DC) are most commonly generated from monocytes using standard cell culture dishes. To elucidate the effect of the plastic surface during the differentiation process, we compared a standard adhesive plastic dish with four different mainly non-adherent surfaces. Untouched monocytes were cultured for 3 days in the presence of IL-4 and GM-CSF. Time-lapse videos were recorded, and the phenotype of the cells was analysed by flow cytometry. The cytokine profiles were analysed using a 25-plex cytokine assay. The use of non-adherent surfaces led to a significant reduction in expression of CD14 and CD38, and a significant increase in expression of CD86 compared to standard culture dishes. Expression levels of DC-SIGN and PD-L2 were reduced significantly on cells cultured on non-adherent surfaces. The cytokine production was independent on the surface used. The surface-mediated priming should therefore be considered when aiming to induce specific immune responses. This is especially important with regard to DC-based immunotherapy, where an adjustment of the surface during the DC generation process might have highly beneficial effects. |
DOI: | doi:10.1111/sji.12246 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1111/sji.12246 |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/sji.12246 |
| DOI: https://doi.org/10.1111/sji.12246 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1700650505 |
Verknüpfungen: | → Zeitschrift |
Surface-mediated priming during in vitro generation of monocyte-derived dendritic cells / Sauter, Alexander [VerfasserIn]; 2015 (Online-Ressource)
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