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Status: Bibliographieeintrag

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Verfasst von:Seol, Min [VerfasserIn]   i
 Kuner, Thomas [VerfasserIn]   i
Titel:Ionotropic glutamate receptor GluA4 and T-type calcium channel Cav3.1 subunits control key aspects of synaptic transmission at the mouse L5B-POm giant synapse
Verf.angabe:Min Seol and Thomas Kuner
E-Jahr:2015
Jahr:22 September 2015
Umfang:12 S.
Fussnoten:Gesehen am 16.06.2020 ; Im Titel ist "v" in Cav3 tiefgestellt
Titel Quelle:Enthalten in: European journal of neuroscience
Ort Quelle:Oxford [u.a.] : Wiley, 1989
Jahr Quelle:2015
Band/Heft Quelle:42(2015), 12, Seite 3033-3044
ISSN Quelle:1460-9568
Abstract:The properties and molecular determinants of synaptic transmission at giant synapses connecting layer 5B (L5B) neurons of the somatosensory cortex (S1) with relay neurons of the posteriomedial nucleus (POm) of the thalamus have not been investigated in mice. We addressed this by using direct electrical stimulation of fluorescently labelled single corticothalamic terminals combined with molecular perturbations and whole-cell recordings from POm relay neurons. Consistent with their function as drivers, we found large-amplitude excitatory postsynaptic currents (EPSCs) and multiple postsynaptic action potentials triggered by a single presynaptic action potential. To study the molecular basis of these two features, ionotropic glutamate receptors and low voltage-gated T-type calcium channels were probed by virus-mediated genetic perturbation. Loss of GluA4 almost abolished the EPSC amplitude, strongly delaying the onset of action potential generation, but maintaining the number of action potentials generated per presynaptic action potential. In contrast, knockdown of the Cav3.1 subunit abrogated the driver function of the synapse at a typical resting membrane potential of −70 mV. However, when depolarizing the membrane potential to −60 mV, the synapse relayed single action potentials. Hence, GluA4 subunits are required to produce an EPSC sufficiently large to trigger postsynaptic action potentials within a defined time window after the presynaptic action potential, while Cav3.1 expression is essential to establish the driver function of L5B-POm synapses at hyperpolarized membrane potentials.
DOI:doi:10.1111/ejn.13084
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1111/ejn.13084
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/ejn.13084
 DOI: https://doi.org/10.1111/ejn.13084
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:cortico-thalamic synapse
 giant synapse
 synaptic transmission
K10plus-PPN:1700667386
Verknüpfungen:→ Zeitschrift

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