| Online-Ressource |
Verfasst von: | Rattay, Kristin [VerfasserIn]  |
| Meyer, Hannah V. [VerfasserIn]  |
| Herrmann, Carl [VerfasserIn]  |
| Brors, Benedikt [VerfasserIn]  |
| Kyewski, Bruno [VerfasserIn]  |
Titel: | Evolutionary conserved gene co-expression drives generation of self-antigen diversity in medullary thymic epithelial cells |
Verf.angabe: | Kristin Rattay, Hannah Verena Meyer, Carl Herrmann, Benedikt Brors, Bruno Kyewski |
E-Jahr: | 2016 |
Jahr: | [2016] |
Umfang: | 11 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Available online 23 October 2015 ; Gesehen am 17.06.2020 |
Titel Quelle: | Enthalten in: Journal of autoimmunity |
Ort Quelle: | London : Academic Press, 1988 |
Band/Heft Quelle: | 67(2016), Seite 65-75 |
ISSN Quelle: | 1095-9157 |
Abstract: | Promiscuous expression of a plethora of tissue-restricted antigens (TRAs) in medullary thymic epithelial cells (mTECs) is essential for central tolerance. This promiscuous gene expression (pGE) is characterized by inclusion of a broad range of TRAs and by its mosaic expression patterns, i.e. each antigen is only expressed in 1-3% of mTECs. It is currently unclear to which extent random and/or deterministic mechanisms are involved in the regulation of pGE. In order to address this issue, we deconstructed the transcriptional heterogeneity in mTEC to minor subsets expressing a particular TRA. We identified six delineable co-expression groups in mouse mTECs. These co-expression groups displayed a variable degree of mutual overlap and mapped to different stages of mTEC development. Co-expressed genes showed chromosomal preference and clustered within delimited genomic regions. Moreover, co-expression groups in mice and humans selected by a pair of orthologous genes preferentially co-expressed sets of orthologous genes attesting to the species conservation of pGE between mouse and human. Furthermore, co-expressed genes were enriched for specific transcription factor binding motifs concomitant with up-regulation of the corresponding transcription factors, implicating additional factors in the regulation of pGE besides the Autoimmune Regulator (Aire). Thus promiscuous transcription of self-antigens in mTECs entails a highly coordinated process, which is evolutionary strictly conserved between species. |
DOI: | doi:10.1016/j.jaut.2015.10.001 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.jaut.2015.10.001 |
| Volltext: http://www.sciencedirect.com/science/article/pii/S0896841115300469 |
| DOI: https://doi.org/10.1016/j.jaut.2015.10.001 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Gene co-expression |
| Medullary thymic epithelial cells |
| Promiscuous gene expression |
| Tissue-restricted antigen |
| Tolerance |
K10plus-PPN: | 1700722395 |
Verknüpfungen: | → Zeitschrift |
Evolutionary conserved gene co-expression drives generation of self-antigen diversity in medullary thymic epithelial cells / Rattay, Kristin [VerfasserIn]; [2016] (Online-Ressource)