Poly-acrylic acid brushes and adsorbed proteins

• Verfasst von: Reinhardt, Matthias [VerfasserIn]
• Verfasst von: Kreuzer, Martin [VerfasserIn]
• Verfasst von: Geue, Thomas [VerfasserIn]
• Verfasst von: Dahint, Reiner [VerfasserIn]
• Verfasst von: Ballauff, Matthias [VerfasserIn]
• Verfasst von: Steitz, Roland [VerfasserIn]
• Titel: Poly-acrylic acid brushes and adsorbed proteins
• Verf.angabe: Matthias Reinhardt, Martin Kreuzer, Thomas Geue, Reiner Dahint, Matthias Ballauff, Roland Steitz
• E-Jahr: 2015
• Jahr: 02.04.2015
• Umfang: 21 S.
• Fussnoten: Gesehen am 17.06.2020
• Titel Quelle: Enthalten in: Zeitschrift für physikalische Chemie
• Ort Quelle: Berlin : De Gruyter, 1954
• Jahr Quelle: 2015
• Band/Heft Quelle: 229(2015), 7-8, Seite 1119-1139
• ISSN Quelle: 2196-7156
• DOI: doi:10.1515/zpch-2014-0540
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1700769383

Abstract

<section class="abstract"><h2 class="abstractTitle text-title my-1" id="d635e2">Abstract</h2><p>Planar polyelectrolyte brushes are prepared by Langmuir-Schaefer - based grafting of perdeuterated (styrene)₄₉-b-(acrylic - acid)₂₂₂ block copolymer (dPS-b-PAA) to dPS pre-coated silicon - supports with grafting density <em>σ</em>_PAA from 0.07 to - 0.11 nm^<em>-</em>2. The structure of the solvent-swollen brushes, - i. e. the volume fraction profile of polymer segments, <em>ϕ</em>_PAA, as a function of altitude <em>z</em> from the grafting - plane into the liquid phase is extracted from neutron reflectivity - measurements. We find that for all cases investigated - <em>ϕ</em>_PAA(<em>z</em>) resembles a Gaussian profile. Although very - condensed, the PAA brushes can be loaded with bovine serum albumin - (BSA). The integral amount of adsorbed BSA scales linearly with - grafting density. We compare our <em>z</em>-resolved volume fraction profile - <em>ϕ</em>_BSA(<em>z</em>) of BSA on PAA brushes with existing literature - on that system. It is found that a cross-over takes place in the - adsorption scheme from ternary compressive, where proteins can - approach the grafting surface only by compressing the brush, to - ternary insertive, where proteins enter the brush with only local - perturbation of the concentration profile, as a function of - <em>R</em>_P/<em>H</em>_max, where <em>R</em>_P is the - Stokes-Radius of the protein, and <em>H</em>_max is the - experimentally determined maximum height of - the brush.</p></section>