Status: Bibliographieeintrag
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| Verfasst von: | Baalmann, Mathis [VerfasserIn]  |
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| | Best, Marcel [VerfasserIn] |
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| | Wombacher, Richard [VerfasserIn] |
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| Titel: | Site-specific protein labeling utilizing lipoic acid ligase (LplA) and bioorthogonal inverse electron demand Diels-Alder reaction |
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| Verf.angabe: | Mathis Baalmann, Marcel Best, Richard Wombacher |
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| E-Jahr: | 2018 |
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| Jahr: | 06 February 2018 |
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| Umfang: | 23 S. |
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| Teil: | year:2018 |
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| | pages:365-387 |
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| | extent:23 |
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| Fussnoten: | Gesehen am 18.06.2020 |
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| Titel Quelle: | Enthalten in: Noncanonical amino acids |
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| Ort Quelle: | New York, NY : Humana Press, 2018 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | (2018), Seite 365-387 |
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| ISBN Quelle: | 978-1-4939-7574-7 |
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| Abstract: | Here, we describe a two-step protocol for selective protein labeling based on enzyme-mediated peptide labeling utilizing lipoic acid ligase (LplA) and bioorthogonal chemistry. The method can be applied to purified proteins, protein in cell lysates, as well as living cells. In a first step a W37V mutant of the lipoic acid ligase (LplAW37V) from Escherichia coli is utilized to ligate a synthetic chemical handle site-specifically to a lysine residue in a 13 amino acid peptide motif—a short sequence that can be genetically expressed as a fusion with any protein of interest. In a second step, a molecular probe can be attached to the chemical handle in a bioorthogonal Diels-Alder reaction with inverse electron demand (DAinv). This method is a complementary approach to protein labeling using genetic code expansion and circumvents larger protein tags while maintaining label specificity, providing experimental flexibility and straightforwardness. |
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| DOI: | doi:10.1007/978-1-4939-7574-7_23 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/978-1-4939-7574-7_23 |
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| | DOI: https://doi.org/10.1007/978-1-4939-7574-7_23 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Bioorthogonal reactions |
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| | Fluorescent probes |
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| | Inverse-electron-demand Diels-Alder cycloaddition |
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| | Ligases |
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| | Site-specific protein labeling |
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| | Tetrazines |
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| K10plus-PPN: | 1701071010 |
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| Verknüpfungen: | → Sammelwerk |
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Site-specific protein labeling utilizing lipoic acid ligase (LplA) and bioorthogonal inverse electron demand Diels-Alder reaction / Baalmann, Mathis [VerfasserIn]; 06 February 2018 (Online-Ressource)
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