Status: Bibliographieeintrag
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| Verfasst von: | Sommer, Stefanie [VerfasserIn]  |
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| | Ritterhoff, Tobias [VerfasserIn] |
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| | Melchior, Frauke [VerfasserIn] |
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| | Mootz, Henning D. [VerfasserIn] |
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| Titel: | A stable chemical SUMO1-Ubc9 conjugate specifically binds as a thioester mimic to the RanBP2-E3 ligase complex |
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| Verf.angabe: | Stefanie Sommer, Tobias Ritterhoff, Frauke Melchior, and Henning D. Mootz |
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| E-Jahr: | 2015 |
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| Jahr: | April 27, 2015 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 19.06.2020 |
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| Titel Quelle: | Enthalten in: ChemBioChem |
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| Ort Quelle: | Weinheim : Wiley-VCH, 2000 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 16(2015), 8, Seite 1183-1189 |
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| ISSN Quelle: | 1439-7633 |
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| Abstract: | Abstract Ubiquitin and ubiquitin-like (Ubl) modifiers such as SUMO are conjugated to substrate proteins by E1, E2, and E3 enzymes. In the presence of an E3 ligase, the E2?Ubl thioester intermediate becomes highly activated and is prone to chemical decomposition, thus making biochemical and structural studies difficult. Here we explored a stable chemical conjugate of the E2 enzyme from the SUMO pathway, Ubc9, with its modifier SUMO1 as a structural analogue of the Ubc9?SUMO1 thioester intermediate, by introducing a triazole linkage by biorthogonal click chemistry. The chemical conjugate proved stable against proteolytic cleavage, in contrast to a Ubc9?SUMO1 isopeptide analogue obtained by auto-SUMOylation. Triazole-linked Ubc9?SUMO1 bound specifically to the preassembled E3 ligase complex RanBP2/RanGAP1*SUMO1/Ubc9, thus suggesting that it is a suitable thioester mimic. We anticipate interesting prospects for its use as a research tool to study protein complexes involving E2 and E3 enzymes. |
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| DOI: | doi:10.1002/cbic.201500011 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/cbic.201500011 |
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| | Volltext: https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201500011 |
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| | DOI: https://doi.org/10.1002/cbic.201500011 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | biological activity |
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| | click chemistry |
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| | enzyme catalysis |
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| | post-translational modifications |
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| | SUMO |
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| | ubiquitin |
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| K10plus-PPN: | 1701118149 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ stable chemical SUMO1-Ubc9 conjugate specifically binds as a thioester mimic to the RanBP2-E3 ligase complex / Sommer, Stefanie [VerfasserIn]; April 27, 2015 (Online-Ressource)
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