Heparin modulates chemokines in human endometrial stromal cells by interaction with tumor necrosis factor α and thrombin

• Verfasst von: Spratte, Julia [VerfasserIn]
• Verfasst von: Schönborn, Magdalena [VerfasserIn]
• Verfasst von: Treder, Nora [VerfasserIn]
• Verfasst von: Bornkessel, Frauke [VerfasserIn]
• Verfasst von: Zygmunt, Marek [VerfasserIn]
• Verfasst von: Fluhr, Herbert [VerfasserIn]
• Titel: Heparin modulates chemokines in human endometrial stromal cells by interaction with tumor necrosis factor α and thrombin
• Verf.angabe: Julia Spratte M.D., Magdalena Schönborn B.S., Nora Treder B.S., Frauke Bornkessel B.S., Marek Zygmunt M.D., Ph.D., Herbert Fluhr M.D., Ph.D.
• E-Jahr: 2015
• Jahr: March 23, 2015
• Umfang: 7 S.
• Fussnoten: Gesehen am 22.06.2020
• Titel Quelle: Enthalten in: Fertility and sterility
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1950
• Jahr Quelle: 2015
• Band/Heft Quelle: 103(2015), 5, Seite 1363-1369
• ISSN Quelle: 1556-5653
• DOI: doi:10.1016/j.fertnstert.2015.02.023
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: chemokines
• Sach-SW: endometrium
• Sach-SW: Heparin
• Sach-SW: thrombin
• Sach-SW: TNF-α
• K10plus-PPN: 1701693666

Abstract

Objective - To study the impact of heparins on chemokines in decidualized human endometrial stromal cells (ESCs) in vitro. - Design - In vitro experiment. - Setting - Research laboratory. - Patient(s) - Premenopausal women undergoing hysterectomy for benign reasons. - Intervention(s) - ESCs were isolated from hysterectomy specimens, decidualized in vitro and incubated with unfractionated heparin and low-molecular-weight heparins (LMWHs) as well as tumor necrosis factor (TNF) α or thrombin with or without heparins. - Main Outcome Measure(s) - Chemokines CXCL1, CXCL5, CXCL8, CCL2, and CCL5 were measured with the use of ELISA, and CXCL5, CXCL8, CCL2, and CCL5 were detected with the use of real-time reverse-transcription polymerase chain reaction. Cell viability was determined with the use of a fluorometric assay. - Result(s) - TNF-α and thrombin stimulated distinct patterns of chemokines in ESCs. Unfractionated heparin and LMWHs attenuated the TNF-α-mediated induction of CXCL8 and enhanced CXCL5, CCL2, and CCL5. The stimulating effect of thrombin on CXCL8 could be inhibited by heparin, whereas heparin had no impact on thrombin-induced CXCL1 and CCL2. Nuclear factor of transcription κB signaling mediated the effects of TNF-α. The effects of thrombin were mediated via extracellular signal-regulated protein kinases 1/2. - Conclusion(s) - Heparins have modulating effects on TNF-α- and thrombin-induced endometrial chemokines, which might have implications in the regulation of endometrial receptivity and early implantation.