Freely drawn single lipid nanotube patterns

• Verfasst von: Sugihara, Kaori [VerfasserIn]
• Verfasst von: Rustom, Amin [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Freely drawn single lipid nanotube patterns
• Verf.angabe: Kaori Sugihara, Amin Rustom and Joachim P. Spatz
• E-Jahr: 2015
• Jahr: 20 Jan 2015
• Umfang: 7 S.
• Fussnoten: Gesehen am 23.06.2020
• Titel Quelle: Enthalten in: Soft matter
• Ort Quelle: London : Royal Soc. of Chemistry, 2005
• Jahr Quelle: 2015
• Band/Heft Quelle: 11(2015), 10, Seite 2029-2035
• ISSN Quelle: 1744-6848
• DOI: doi:10.1039/C5SM00043B
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1701936933

Abstract

LNTs are unique 3D structures made only of safe and abundant biomaterials by self-assembly. The current bottleneck for developing applications using LNTs is the lack of an easy technique to pattern them on substrates. We report a method to free-draw single lipid nanotube (LNT) patterns in any shape on surfaces with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) that takes an inverted hexagonal (HII) phase. We used pre-self-assembled LNTs or HII lipid blocks as a lipid reservoir from which new LNTs were pulled by applying a point load with a micromanipulator. The extreme simplicity of our technique originates from the fundamental nature of DOPE lipids that prefer a HII phase, while all the conventional approaches use PC lipids that form a lamellar phase. By adjusting the surface properties with polyelectrolyte multilayers, the created single LNT objects are able to remain adhered to the surface for over a week. Importantly, it could be shown that two vesicles loaded with caged fluorescent molecules were able to fuse well with a LNT, enabling diffusive transport of uncaged fluorescent molecules from one vesicle to the other.