Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Rimbach, Katharina [VerfasserIn]  |
| Kaiser, Steffen [VerfasserIn]  |
| Helm, Mark [VerfasserIn]  |
| Dalpke, Alexander [VerfasserIn]  |
| Eigenbrod, Tatjana [VerfasserIn]  |
Titel: | 2'-O-methylation within bacterial RNA acts as suppressor of TLR7/TLR8 activation in human innate immune cells |
Verf.angabe: | Katharina Rimbach, Steffen Kaiser, Mark Helm, Alexander H. Dalpke, Tatjana Eigenbrod |
E-Jahr: | 2015 |
Jahr: | March 20, 2015 |
Umfang: | 12 S. |
Fussnoten: | Gesehen am 25.06.2020 |
Titel Quelle: | Enthalten in: Journal of innate immunity |
Ort Quelle: | Basel : Karger, 2009 |
Jahr Quelle: | 2015 |
Band/Heft Quelle: | 7(2015), 5, Seite 482-493 |
ISSN Quelle: | 1662-8128 |
Abstract: | Microbial RNA is an important stimulator of innate immune responses. Differences in posttranscriptional RNA modification profiles enable the immune system to discriminate between self and non-self nucleic acids. This principle may be exploited by certain bacteria to circumvent immune cell activation. In this regard, 2'-O-methylation of <i>Escherichia coli</i> tRNA<sup>Tyr</sup> at position 18 (Gm18) has recently been described to inhibit TLR7-mediated IFN-a production in human plasmacytoid dendritic cells (pDCs). Extending these findings, we now demonstrate that Gm18 also potently inhibits TLR7-independent human monocyte activation by RNA derived from a variety of bacterial strains. The half minimal inhibitory concentration values were similar to those found for IFN-a inhibition in pDCs. Mechanistically, 2'-O-methylated RNA impaired upstream signalling events, including MAP kinase and NFκB activation. Our results suggest that antagonizing effects of Gm18-modified RNA are due to competition with stimulatory RNA for receptor binding. The antagonistic effect was specific for RNA because the small molecule TLR7/8 agonist R848 was not inhibited. Despite the striking phenotype in human cells, 2'-O-methylated RNA did not interfere with TLR13 activation by bacterial 23S rRNA in murine DC and BMDM. Thus, we identify here Gm18 in <i>E. coli</i> tRNA<sup>Tyr</sup> as a universal suppressor of innate immune activation in the human but not the murine system. |
DOI: | doi:10.1159/000375460 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1159/000375460 |
| Volltext: https://www.karger.com/Article/FullText/375460 |
| DOI: https://doi.org/10.1159/000375460 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1702126471 |
Verknüpfungen: | → Zeitschrift |
2'-O-methylation within bacterial RNA acts as suppressor of TLR7/TLR8 activation in human innate immune cells / Rimbach, Katharina [VerfasserIn]; March 20, 2015 (Online-Ressource)
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