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Verfasst von:Rimbach, Katharina [VerfasserIn]   i
 Kaiser, Steffen [VerfasserIn]   i
 Helm, Mark [VerfasserIn]   i
 Dalpke, Alexander [VerfasserIn]   i
 Eigenbrod, Tatjana [VerfasserIn]   i
Titel:2'-O-methylation within bacterial RNA acts as suppressor of TLR7/TLR8 activation in human innate immune cells
Verf.angabe:Katharina Rimbach, Steffen Kaiser, Mark Helm, Alexander H. Dalpke, Tatjana Eigenbrod
E-Jahr:2015
Jahr:March 20, 2015
Umfang:12 S.
Fussnoten:Gesehen am 25.06.2020
Titel Quelle:Enthalten in: Journal of innate immunity
Ort Quelle:Basel : Karger, 2009
Jahr Quelle:2015
Band/Heft Quelle:7(2015), 5, Seite 482-493
ISSN Quelle:1662-8128
Abstract:Microbial RNA is an important stimulator of innate immune responses. Differences in posttranscriptional RNA modification profiles enable the immune system to discriminate between self and non-self nucleic acids. This principle may be exploited by certain bacteria to circumvent immune cell activation. In this regard, 2'-O-methylation of <i>Escherichia coli</i> tRNA<sup>Tyr</sup> at position 18 (Gm18) has recently been described to inhibit TLR7-mediated IFN-a production in human plasmacytoid dendritic cells (pDCs). Extending these findings, we now demonstrate that Gm18 also potently inhibits TLR7-independent human monocyte activation by RNA derived from a variety of bacterial strains. The half minimal inhibitory concentration values were similar to those found for IFN-a inhibition in pDCs. Mechanistically, 2'-O-methylated RNA impaired upstream signalling events, including MAP kinase and NF&#954;B activation. Our results suggest that antagonizing effects of Gm18-modified RNA are due to competition with stimulatory RNA for receptor binding. The antagonistic effect was specific for RNA because the small molecule TLR7/8 agonist R848 was not inhibited. Despite the striking phenotype in human cells, 2'-O-methylated RNA did not interfere with TLR13 activation by bacterial 23S rRNA in murine DC and BMDM. Thus, we identify here Gm18 in <i>E. coli</i> tRNA<sup>Tyr</sup> as a universal suppressor of innate immune activation in the human but not the murine system.
DOI:doi:10.1159/000375460
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1159/000375460
 Volltext: https://www.karger.com/Article/FullText/375460
 DOI: https://doi.org/10.1159/000375460
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1702126471
Verknüpfungen:→ Zeitschrift

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