Status: Bibliographieeintrag
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| Verfasst von: | Wabnitz, Guido H. [VerfasserIn]  |
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| | Neßmann, Anja [VerfasserIn] |
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| | Kirchgessner, Henning [VerfasserIn] |
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| | Samstag, Yvonne [VerfasserIn] |
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| Titel: | InFlow microscopy of human leukocytes |
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| Titelzusatz: | a tool for quantitative analysis of actin rearrangements in the immune synapse |
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| Verf.angabe: | Guido H. Wabnitz, Anja Nessmann, Henning Kirchgessner, Yvonne Samstag |
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| E-Jahr: | 2015 |
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| Jahr: | 17 March 2015 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 25.06.2020 |
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| Titel Quelle: | Enthalten in: Journal of immunological methods |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1971 |
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| Jahr Quelle: | 2015 |
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| Band/Heft Quelle: | 423(2015), Seite 29-39 |
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| ISSN Quelle: | 1872-7905 |
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| Abstract: | The actin cytoskeleton is a main component to preserve the cell shape. It represents a cellular machinery that enables morphological changes and orchestrates important dynamic cellular functions. Thereby, it supports T-cell migration, immune synapse formation, activation and execution of effector functions. The analysis of actin rearrangements in T-cells is therefore an important field of basic and clinical research. Actin reorganization is traditionally performed using flow cytometry or confocal microscopy. However, while flow cytometry lacks spatial and structural information, confocal microscopy is time consuming and not feasible for the characterization of rare events or of un-purified primary cell populations. Here we describe a methodology to analyze actin rearrangements using InFlow microscopy, which is a hybrid technique consisting of flow cytometric and microscopic features. We show that InFlow microscopy is a valuable tool for quantification of the amount and distribution of F-actin in human T-cells after stimulation with chemokines or antigen-presenting cells. |
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| DOI: | doi:10.1016/j.jim.2015.03.003 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.jim.2015.03.003 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0022175915000678 |
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| | DOI: https://doi.org/10.1016/j.jim.2015.03.003 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Actin cytoskeleton |
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| | Imaging flow cytometry |
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| | Immune synapse |
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| | T-cells |
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| K10plus-PPN: | 1702150127 |
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| Verknüpfungen: | → Zeitschrift |
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InFlow microscopy of human leukocytes / Wabnitz, Guido H. [VerfasserIn]; 17 March 2015 (Online-Ressource)
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