Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Nöske, Katharina [VerfasserIn]   i
 Stark, Hans-Jürgen [VerfasserIn]   i
 Nevaril, Leonard [VerfasserIn]   i
 Berning, Manuel [VerfasserIn]   i
 Langbein, Lutz [VerfasserIn]   i
 Goyal, Ashish [VerfasserIn]   i
 Diederichs, Sven [VerfasserIn]   i
 Boukamp, Petra [VerfasserIn]   i
Titel:Mitotic diversity in homeostatic human interfollicular epidermis
Verf.angabe:Katharina Nöske, Hans-Jürgen Stark, Leonard Nevaril, Manuel Berning, Lutz Langbein, Ashish Goyal, Sven Diederichs and Petra Boukamp
E-Jahr:2016
Jahr:28 January 2016
Umfang:16 S.
Fussnoten:Gesehen am 26.06.2020
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2016
Band/Heft Quelle:17(2016,2) Artikel-Nummer 167, 16 Seiten
ISSN Quelle:1422-0067
 1661-6596
Abstract:Despite decades of skin research, regulation of proliferation and homeostasis in human epidermis is still insufficiently understood. To address the role of mitoses in tissue regulation, we utilized human long-term skin equivalents and systematically assessed mitoses during early epidermal development and long-term epidermal regeneration. We now demonstrate four different orientations: (1) horizontal, i.e., parallel to the basement membrane (BM) and suggestive of symmetric divisions; (2) oblique with an angle of 45°-70°; or (3) perpendicular, suggestive of asymmetric division. In addition, we demonstrate a fourth substantial fraction of suprabasal mitoses, many of which are committed to differentiation (Keratin K10-positive). As verified also for normal human skin, this spatial mitotic organization is part of the regulatory program of human epidermal tissue homeostasis. As a potential marker for asymmetric division, we investigated for Numb and found that it was evenly spread in almost all undifferentiated keratinocytes, but indeed asymmetrically distributed in some mitoses and particularly frequent under differentiation-repressing low-calcium conditions. Numb deletion (stable knockdown by CRISPR/Cas9), however, did not affect proliferation, neither in a three-day follow up study by life cell imaging nor during a 14-day culture period, suggesting that Numb is not essential for the general control of keratinocyte division.
DOI:doi:10.3390/ijms17020167
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3390/ijms17020167
 Volltext: https://www.mdpi.com/1422-0067/17/2/167
 DOI: https://doi.org/10.3390/ijms17020167
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:asymmetric mitosis
 epidermal differentiation
 human epidermis
 long-term organotypic culture model
 Numb
 suprabasal mitosis
K10plus-PPN:1702652017
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68591634   QR-Code
zum Seitenanfang